Instituto de Pesquisa e Desenvolvimento - IP&D, Universidade do Vale do Paraíba - UNIVAP, São José dos Campos, Brazil.
J Biomater Sci Polym Ed. 2020 Dec;31(17):2182-2198. doi: 10.1080/09205063.2020.1795461. Epub 2020 Jul 21.
It has been demonstrated an increase in resistance of to conventional therapies, probably, due the indiscriminate use of the conventional antifungal drugs. In this aspect, the nanotechnology generates the possibility of creating new therapeutic agents. Thus, the objective of this paper was to produce and characterize a bovine serum albumin (BSA) nanoparticle encapsulated with Methylene Blue (MB). In addition, the effect of BSA nanoparticles encapsulated with MB (BSA-MB) was evaluated on both growth and biofilm formation by by Photodynamic Antimicrobial Chemotherapy (PACT) protocols. The BSA-MB nanoparticles were prepared by the desolvation process. The nanoparticulate system was studied by steady-state techniques, scanning electron microscopy and their biological activity was evaluated both growth and biofilm formation by . The synthetized BSA-MB nanoparticles were spherical in shape exhibiting a 100-200 nm diameter with a low tendency to aggregate (PDI values < 0.2). MB photophysical properties were shown to be preserved after BSA encapsulation. A significant reduction in growth, after PACT was observed, in a dependent manner on MB-loaded in BSA nanoparticles concentration used. It was observed an inhibition of 23, 65 and 83% in the presence of MB-loaded in BSA nanoparticles 0.1, 0.5 and 1.0 µg.mL, respectively. In addition, MB-loaded BSA nanoparticles 0.5 µg.mL were able to reduce both biofilm formation (80%) and the transition from yeast to filamentous form by . The results presented here demonstrated a potentiation of the phototoxic effect of MB after BSA encapsulation, since the concentrations of MB-loaded BSA nanoparticles necessary to inhibits ∼50% of development was 10 times minor than that observed for free MB. Taken together, these results suggest the potential of PACT, using MB-loaded BSA nanoparticles in inhibiting development. The synthesis and design of BSA nanoparticles can be successfully applied for MB encapsulation and offer the possibility to drive the toxicity effect to a specific target, as an evaluation on both growth and biofilm formation by .
已经证明，由于常规抗真菌药物的滥用，对传统疗法的耐药性有所增加。在这方面，纳米技术为创造新的治疗剂提供了可能性。因此，本文的目的是生产和表征一种牛血清白蛋白（BSA）包裹亚甲蓝（MB）的纳米颗粒。此外，还通过光动力抗菌化疗（PACT）方案评估了包裹 MB 的 BSA 纳米颗粒（BSA-MB）对生长和生物膜形成的影响。BSA-MB 纳米颗粒通过去溶剂化过程制备。通过稳态技术、扫描电子显微镜研究了纳米颗粒体系，评估了合成的 BSA-MB 纳米颗粒对生长和生物膜形成的生物活性。所合成的 BSA-MB 纳米颗粒呈球形，粒径为 100-200nm，具有低聚集倾向（PDI 值<0.2）。BSA 包封后，MB 的光物理性质得以保留。在 PACT 后观察到，在依赖于所用 BSA 纳米颗粒中负载的 MB 浓度的情况下，对生长的抑制作用呈依赖性。当存在 0.1、0.5 和 1.0μg.mL 的 BSA 纳米颗粒中负载的 MB 时，观察到 23、65 和 83%的抑制作用。此外，BSA 纳米颗粒中负载的 MB 0.5μg.mL 能够减少生物膜形成（80%）和通过 向丝状形态的转变。这里呈现的结果表明，在 BSA 包封后，MB 的光毒性作用得到增强，因为抑制生长的 MB-负载 BSA 纳米颗粒所需的浓度是观察到游离 MB 的 10 倍。综上所述，这些结果表明，使用 BSA 纳米颗粒中负载的 MB 的 PACT 在抑制 生长方面具有潜力。BSA 纳米颗粒的合成和设计可成功应用于 MB 包封，并提供将毒性作用靶向特定目标的可能性，如对生长和生物膜形成的评估。
