Meng Lu, Liu Huimin, Lan Tu, Dong Lei, Hu Haiyan, Zhao Shengguo, Zhang Yangdong, Zheng Nan, Wang Jiaqi
Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2020 Jun 3;11:1005. doi: 10.3389/fmicb.2020.01005. eCollection 2020.
Psychrotrophic bacteria in raw milk are most well known for their spoilage potential and the economic losses they cause to the dairy industry. Food-related psychrotrophic bacteria are increasingly reported to have antibiotic resistance features. The aim of this study was to evaluate the resistance patterns of spp. isolated from bulk-tank milk. In total, we investigated the antibiotic susceptibility profiles of 86 spp. isolates from raw milk. All strains were tested against 15 antimicrobial agents. isolates were most highly resistant to imipenem (95.3%), followed by trimethoprim-sulfamethoxazole (69.8%), aztreonam (60.5%), chloramphenicol (45.3%), and meropenem (27.9%). Their multiple antibiotic resistance (MAR) index values ranged from 0.0 to 0.8. Whole-genome sequencing revealed the presence of intrinsic resistance determinants, such as BcI, C-09, CTX-M, D, 1, E, A1, B3, I, R, and V. Moreover, resistance-nodulation-cell division (RND) and ATP-binding cassette (ABC) antibiotic efflux pumps were also found. This study provides further knowledge of the antibiotic resistance patterns of spp. in milk, which may advance our understanding of resistance in and suggests that antibiotic resistance of spp. in raw milk should be a concern.
生牛奶中的嗜冷菌因其潜在的腐败作用以及给乳制品行业造成的经济损失而最为人所知。越来越多的报道称，与食品相关的嗜冷菌具有抗生素耐药特性。本研究的目的是评估从大容量储存罐牛奶中分离出的[菌名未明确给出]菌株的耐药模式。我们总共调查了86株从生牛奶中分离出的[菌名未明确给出]菌株的抗生素敏感性概况。所有菌株都针对15种抗菌剂进行了测试。[菌名未明确给出]菌株对亚胺培南的耐药性最高（95.3%），其次是甲氧苄啶 - 磺胺甲恶唑（69.8%）、氨曲南（60.5%）、氯霉素（45.3%）和美罗培南（27.9%）。它们的多重抗生素耐药（MAR）指数值范围为0.0至0.8。全基因组测序揭示了内在耐药决定因素的存在，如BcI、C - 09、CTX - M、D、1、E、A1、B3、I、R和V。此外，还发现了耐药 - 结瘤 - 细胞分裂（RND）和ATP结合盒（ABC）抗生素外排泵。本研究进一步提供了牛奶中[菌名未明确给出]菌株抗生素耐药模式的知识，这可能会增进我们对[菌名未明确给出]耐药性的理解，并表明生牛奶中[菌名未明确给出]菌株的抗生素耐药性应受到关注。
