Saini Kritika, Kaushal Ankur, Gupta Shagun, Kumar Dinesh
Shoolini University of Biotechnology and Management Sciences, Bajhol, PO, Distt. Solan, Sultanpur, 173229 HP India.
Amity University, Manesar, Gurugram, 122413 Haryana India.
3 Biotech. 2020 Jul;10(7):327. doi: 10.1007/s13205-020-02315-0. Epub 2020 Jul 1.
The electrochemical DNA biosensor has been developed for the detection of in raw milk samples. The electrochemical studies of the developed biosensor was recorded by cyclic voltammetry (CV) and electrochemical impedance (EI) using methylene blue (MB) and potassium ferricyanide KFe(CN) as redox indicators. The selectivity of the developed biosensor was demonstrated using complementary and mismatch oligonucleotide sequences. The sensitivity (S) of the developed sensor was recorded as 3461 (μA/cm)/ng and limit of detection (LOD) was found to be 82 fg/6 µl with the regression coefficient ( ) 0.941 using CV. The sensor was characterized by field emission scanning electron microscopy (FE-SEM). The electrode was found to be stable for six months, with only 10% loss in the initial CV current.
已经开发出用于检测生乳样品中[具体物质未给出]的电化学DNA生物传感器。使用亚甲基蓝（MB）和铁氰化钾KFe(CN) 作为氧化还原指示剂，通过循环伏安法（CV）和电化学阻抗（EI）记录所开发生物传感器的电化学研究。使用互补和错配的寡核苷酸序列证明了所开发生物传感器的选择性。使用CV时，所开发传感器的灵敏度（S）记录为3461（μA/cm）/ng，检测限（LOD）为82 fg/6 μl，回归系数（）为0.941。通过场发射扫描电子显微镜（FE-SEM）对该传感器进行了表征。发现该电极在六个月内稳定，初始CV电流仅损失10%。
