Noce Annalisa, Bocedi Alessio, Campo Margherita, Marrone Giulia, Di Lauro Manuela, Cattani Giada, Di Daniele Nicola, Romani Annalisa
UOC of Internal Medicine-Center of Hypertension and Nephrology Unit, Department of Systems Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy.
Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy.
Pharmaceuticals (Basel). 2020 Jul 10;13(7):148. doi: 10.3390/ph13070148.
The identification of natural bioactive compounds, able to counteract the abnormal increase of oxidative stress and inflammatory status in chronic degenerative non-communicable diseases is useful for the clinical management of these conditions. We tested an oral food supplement (OFS), chemically characterized and evaluated for in vitro and in vivo activity. Vitamin C, analyzed by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD), was 0.19 mg/g in rosehip dry extract and 15.74 mg/capsule in the OFS. The identification of polyphenols was performed by HPLC-DAD; the total antioxidant capacity was assessed by Folin-Ciocalteu test. Total polyphenols were 14.73 mg/g gallic acid equivalents (GAE) for rosehip extract and 1.93 mg/g GAE for OFS. A total of 21 chronic kidney disease (CKD) patients and 10 healthy volunteers were recruited. The evaluation of routine laboratory and inflammatory parameters, erythrocyte glutathione transferase (e-GST), human oxidized serum albumin (HSAox), and assessment of body composition were performed at two different times, at baseline and after 5 weeks of OFS assumption. In the study, we highlighted a significant decrease of traditional inflammatory biomarkers (such as C-reactive protein, erythrocyte sedimentation rate, platelet to lymphocyte ratio) and other laboratory parameters like e-GST, azotaemia, and albuminuria after OFS treatment in CKD patients. Moreover, we demonstrated a lipid profile improvement in CKD patients after OFS supplementation.
鉴定能够对抗慢性退行性非传染性疾病中氧化应激和炎症状态异常增加的天然生物活性化合物，对这些疾病的临床管理很有用。我们测试了一种口服食品补充剂（OFS），对其进行了化学表征并评估了体外和体内活性。通过高效液相色谱 - 二极管阵列检测器（HPLC - DAD）分析，玫瑰果干提取物中维生素C含量为0.19 mg/g，OFS中为15.74 mg/胶囊。通过HPLC - DAD进行多酚鉴定；通过福林 - 西奥尔特试验评估总抗氧化能力。玫瑰果提取物中总多酚含量为14.73 mg/g没食子酸当量（GAE），OFS中为1.93 mg/g GAE。共招募了21名慢性肾脏病（CKD）患者和10名健康志愿者。在两个不同时间，即基线时和服用OFS 5周后，进行常规实验室和炎症参数、红细胞谷胱甘肽转移酶（e - GST）、人氧化血清白蛋白（HSAox）的评估以及身体成分评估。在该研究中，我们强调了CKD患者在接受OFS治疗后，传统炎症生物标志物（如C反应蛋白、红细胞沉降率、血小板与淋巴细胞比率）以及其他实验室参数如e - GST、氮血症和蛋白尿显著降低。此外，我们证明了CKD患者在补充OFS后血脂状况得到改善。
