Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Chemistry, University College London, Kathleen Lonsdale Materials Chemistry, London, UK.
Microsc Res Tech. 2020 Nov;83(11):1308-1320. doi: 10.1002/jemt.23522. Epub 2020 Jul 14.
In the present study, green silver nanoparticles (Ag ONPs) were prepared from aqueous and ethanolic leaves extract of Rhamnus virgata in a facile, green, cost-effective, and eco-friendly way. The color changes from light brown to brownish black determined the synthesis of Ag ONPs and Ag ONPs . The phytofabrication of Ag ONPs was confirmed using various spectroscopic and microscopic techniques: energy-dispersive X-ray spectroscopy, dynamic light scattering, ultraviolet-visible spectroscopy, Fourier-transform infrared, X-ray powder diffraction, Raman, scanning electron microscopy, and transmission electron microscopy. Detailed in vitro biological activities determined significant biopotentials for Ag ONPs. The Ag ONPs and Ag ONPs were investigated for anticancer potential against HUH-7 (IC : 9.075 μg/ml for Ag O and 25.66 μg/ml for Ag O ) and HepG2 (IC : 25.18 μg/ml for Ag O and IC : 27.74 μg/ml for Ag O ) cell lines. Concentration-dependent cytotoxicity was performed against brine-shrimps (IC : 36.04 μg/ml for Ag O and 28.82 μg/ml for Ag O ) and Leishmanial parasite (amastigotes and promastigotes). Disc-diffusion method revealed significant antimicrobial activities. In addition, significant enzyme inhibitory activity and antiradical potentials were studied. The hemocompatible nature of Ag ONPs and Ag ONPs was revealed using biocompatibility tests. In conclusion, the green Ag ONPs and Ag ONPs are nontoxic and biocompatible and has shown significant biological activities. We further encourage in vivo studies to ensure biosafety and biocompatibility, so that they can be effectively utilized in nano-pharmaceutical industries.
在本研究中，从 Rhamnus virgata 的水提物和醇提物中通过简便、绿色、经济和环保的方法制备了绿色的银纳米粒子（AgONPs）。颜色从浅棕色变为棕黑色确定了 AgONPs 的合成和 AgONPs 的形成。AgONPs 的植物合成通过各种光谱和显微镜技术得到了证实：能量色散 X 射线光谱、动态光散射、紫外可见光谱、傅里叶变换红外光谱、X 射线粉末衍射、拉曼光谱、扫描电子显微镜和透射电子显微镜。详细的体外生物活性研究确定了 AgONPs 的显著生物潜力。AgONPs 和 AgONPs 被用于研究其对 HUH-7（AgO 的 IC 为 9.075μg/ml，AgO 的 IC 为 25.66μg/ml）和 HepG2（AgO 的 IC 为 25.18μg/ml，AgO 的 IC 为 27.74μg/ml）细胞系的抗癌潜力。对盐水虾（AgO 的 IC 为 36.04μg/ml，AgO 的 IC 为 28.82μg/ml）和利什曼原虫（无鞭毛体和前鞭毛体）进行了浓度依赖性的细胞毒性试验。圆盘扩散法显示出显著的抗菌活性。此外，还研究了其酶抑制活性和抗自由基活性。AgONPs 和 AgONPs 的血液相容性通过生物相容性试验得到了揭示。总之，绿色的 AgONPs 和 AgONPs 具有非毒性和生物相容性，并表现出显著的生物活性。我们进一步鼓励进行体内研究，以确保生物安全性和生物相容性，从而使它们能够有效地应用于纳米制药工业。
