Gad Karol, Huu Cuong Nguyen, Plaschil Sylvia, Kappel Christian, Lenhard Michael
Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany.
Institute for Breeding Research on Horticultural Crops, Julius Kühn Institute - Federal Research Centre for Cultivated Plants, Quedlinburg, Germany.
Front Plant Sci. 2025 Jul 25;16:1623387. doi: 10.3389/fpls.2025.1623387. eCollection 2025.
E. Morren is a widely cultivated ornamental plant with horticultural and pharmacological value. However, the lack of a developed transformation system has limited genetic studies and biotechnological applications of this species. In this study, we established a transformation method using for the genetic manipulation of . The protocol consists of two stages: initial hairy root transformation and subsequent whole-plant regeneration from transgenic hairy roots through somatic embryogenesis. Comprehensive molecular analyses confirmed the stable integration and expression of various transgenes from the root-inducing (Ri) plasmid and the binary vector carrying the reporter in independent transgenic lines, as well as the stable germline transmission of the transgene to progeny. The protocol is effective, with 5% of treated explants successfully forming transformed hairy roots expressing the reporter, from which transgenic plants were regenerated. The established method provides a valuable tool for genetic and molecular studies of heterostyly and the self-incompatibility system in the genus , while also offering practical applications in molecular breeding and plant biotechnology. Additionally, hairy root cultures provide a platform for metabolic engineering and the exploration of biologically active secondary metabolites with pharmacological applications.
E. Morren是一种广泛种植的具有园艺和药用价值的观赏植物。然而，缺乏完善的转化系统限制了该物种的遗传研究和生物技术应用。在本研究中，我们建立了一种用于[物种名称]基因操作的转化方法。该方案包括两个阶段：初始毛状根转化以及随后通过体细胞胚胎发生从转基因毛状根再生完整植株。全面的分子分析证实了来自发根诱导（Ri）质粒和携带[报告基因名称]报告基因的二元载体的各种转基因在独立转基因系中的稳定整合和表达，以及转基因向后代的稳定种系传递。该方案是有效的，5%的处理外植体成功形成表达[报告基因名称]报告基因的转化毛状根，从中再生出转基因植株。所建立的方法为[属名称]中异型花柱和自交不亲和系统的遗传和分子研究提供了有价值的工具，同时也在分子育种和植物生物技术中具有实际应用。此外，毛状根培养为代谢工程和探索具有药理学应用的生物活性次生代谢产物提供了一个平台。
