Paolis Angelo De, Frugis Giovanna, Giannino Donato, Iannelli Maria Adelaide, Mele Giovanni, Rugini Eddo, Silvestri Cristian, Sparvoli Francesca, Testone Giulio, Mauro Maria Luisa, Nicolodi Chiara, Caretto Sofia
Istituto di Scienze delle Produzioni Alimentari (ISPA), Consiglio Nazionale delle Ricerche (CNR), Via Monteroni, 73100 Lecce, Italy.
Istituto di Biologia e Biotecnologia Agraria (IBBA), UOS Roma, Consiglio Nazionale delle Ricerche (CNR), Via Salaria Km. 29,300, Monterotondo Scalo, 00015 Roma, Italy.
Plants (Basel). 2019 Jan 10;8(1):18. doi: 10.3390/plants8010018.
This review is dedicated to the memory of Prof. Domenico Mariotti, who significantly contributed to establishing the Italian research community in Agricultural Genetics and carried out the first experiments of -mediated plant genetic transformation and regeneration in Italy during the 1980s. Following his scientific interests as guiding principles, this review summarizes the recent advances obtained in plant biotechnology and fundamental research aiming to: (i) Exploit in vitro plant cell and tissue cultures to induce genetic variability and to produce useful metabolites; (ii) gain new insights into the biochemical function of genes and their application to metabolite production, fruit tree transformation, and reverse genetics; (iii) improve genetic transformation in legume species, most of them recalcitrant to regeneration; (iv) untangle the potential of KNOTTED1-like homeobox (KNOX) transcription factors in plant morphogenesis as key regulators of hormonal homeostasis; and (v) elucidate the molecular mechanisms of the transition from juvenility to the adult phase in tree species.
本综述谨献给多梅尼科·马里奥蒂教授，他为意大利农业遗传学研究团体的建立做出了重大贡献，并于20世纪80年代在意大利开展了首次介导的植物遗传转化和再生实验。以他的科学兴趣为指导原则，本综述总结了植物生物技术和基础研究方面的最新进展，旨在：（i）利用植物体外细胞和组织培养诱导遗传变异并生产有用的代谢产物；（ii）深入了解基因的生化功能及其在代谢产物生产、果树转化和反向遗传学中的应用；（iii）改进豆科植物的遗传转化，其中大多数植物再生困难；（iv）阐明类KNOTTED1同源异型框（KNOX）转录因子作为激素稳态关键调节因子在植物形态发生中的潜力；以及（v）阐明树种从幼年阶段向成年阶段转变的分子机制。
