Watson P G, Duerden B I
J Clin Pathol. 1977 Jun;30(6):532-6. doi: 10.1136/jcp.30.6.532.
Three methods of preserving simulated specimens of urine were studied with six test strains of bacteria. Viable counts were measured by a surface viable count and by the filter-paper-strip method during a holding period of 72 hours. Refrigeration at approximately 4 degrees C was effective and reliable. Boric acid (1-8%) at room temperature was toxic for the strain of Escherichia coli at a density of 10(7) cfu/ml but this may not be significant at the higher concentration of bacterial cells often found in clinical specimens. NaCl-polyvinylpyrrolidone (PVP) solutions containing PVP of mol. wt 44 000 or 700 000 were not effective; they were toxic for the Gram-negative strains and did not retard the growth of Micrococcus subgroup 3. The two methods of measuring viable counts were compared for specimens held under different conditions; the specificity of the filter-paper-strip method was high but the sensitivity was low when many of the specimens contained approximately 10(5) cfu/ml.
用六种细菌测试菌株研究了三种保存尿液模拟标本的方法。在72小时的保存期内，通过表面活菌计数法和滤纸条法测量活菌数。约4℃冷藏有效且可靠。室温下1 - 8%的硼酸对密度为10(7) cfu/ml的大肠杆菌菌株有毒，但在临床标本中常见的较高细菌细胞浓度下，这可能并不显著。含有分子量为44000或700000的聚乙烯吡咯烷酮（PVP）的氯化钠 - 聚乙烯吡咯烷酮（PVP）溶液无效；它们对革兰氏阴性菌株有毒，且不能抑制3型微球菌的生长。比较了在不同条件下保存的标本的两种活菌计数方法；当许多标本含有约10(5) cfu/ml时，滤纸条法的特异性高但灵敏度低。
