Yamthe Lauve Rachel Tchokouaha, Fokou Patrick Valere Tsouh, Mbouna Cedric Derick Jiatsa, Keumoe Rodrigue, Ndjakou Bruno Lenta, Djouonzo Paul Toukam, Mfopa Alvine Ngoutane, Legac Jennifer, Tsabang Nole, Gut Jiri, Rosenthal Philip J, Boyom Fabrice Fekam
Laboratory for Phytobiochemistry and Medicinal Plants Studies, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O. Box 6163, Yaoundé, Cameroon.
Medicines (Basel). 2015 Apr 30;2(2):55-66. doi: 10.3390/medicines2020055.
The aim of this work was to screen extracts from and against . Crude ethanolic extracts, methylene chloride fractions, aqueous fractions, subfractions and isolated compounds (stigmasterol-3--β-d-glucopyranoside, lichexanthone, gallic acid and β-sitosterol-3--β-d-glucopyranoside) were tested for cytotoxicity on erythrocytes and Human Foreskin Fibroblasts cells and against the W2 strain of in culture. Results indicated that none of the extracts was cytotoxic at concentrations up to 10 µg/mL. Most of the extracts, fractions and subfractions inhibited the growth of with IC values ranging from 0.07 to 3.46 µg/mL. The most potent was the subfraction 30 from stem bark (IC = 0.07 µg/mL) with a selectivity index of ˃ 142. Subfraction 3 from root also exhibited very good activity (IC = 0.09 µg/mL) with a high selectivity index (SI ˃ 111). Amongst the isolated compounds, only gallic acid showed activity with IC of 3.32 µg/mL and SI > 10. These results support traditional claims for and in the treatment of malaria. Given their limited cytotoxicity profile, their extracts qualify as promising starting points for antimalarial drug discovery.
本研究的目的是筛选[植物名称1]和[植物名称2]的提取物对[病原体名称]的作用。对粗乙醇提取物、二氯甲烷馏分、水馏分、亚馏分和分离出的化合物（豆甾醇-3-O-β-D-吡喃葡萄糖苷、地衣黄酮、没食子酸和β-谷甾醇-3-O-β-D-吡喃葡萄糖苷）进行了红细胞和人包皮成纤维细胞的细胞毒性测试以及对培养中的[病原体名称]W2菌株的测试。结果表明，在浓度高达10μg/mL时，没有一种提取物具有细胞毒性。大多数提取物、馏分和亚馏分抑制了[病原体名称]的生长，IC值范围为0.07至3.46μg/mL。最有效的是[植物名称1]茎皮的亚馏分30（IC = 0.07μg/mL），选择性指数大于142。[植物名称2]根的亚馏分3也表现出非常好的活性（IC = 0.09μg/mL），选择性指数高（SI大于111）。在分离出的化合物中，只有没食子酸表现出活性，IC为3.32μg/mL，SI>10。这些结果支持了[植物名称1]和[植物名称2]在治疗疟疾方面的传统说法。鉴于它们有限的细胞毒性特征，它们的提取物有资格作为抗疟药物发现的有前景的起点。
