Duansak Naphatsanan, Piyabhan Pritsana, Srisawat Umarat, Naowaboot Jarinyaporn, Lerdvuthisopon Nusiri, Schmid-Schönbein Geert
Division of Physiology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
Division of Pharmacology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
J Nutr Metab. 2020 Jun 26;2020:8374287. doi: 10.1155/2020/8374287. eCollection 2020.
Inflammation and hypertension are primary mechanisms involving in obesity-associated adverse effects of a high-fat diet. The aim of this study was to evaluate the effects of rice bran extract (RBE) on arterial blood pressure, hepatic steatosis, inflammation, and oxidative stress in high-fat diet (HFD)-induced obese mice.
Male ICR mice were divided into four groups, including a normal-diet control group, a high-fat diet (HFD) (60% kcal from fat) group, an HFD group treated with RBE (220 mg/kg/day), and an HFD group treated with 1100 mg/kg/day for eight weeks. Besides body weight and arterial blood pressure, we determined liver values of total cholesterol, triglyceride, as well as percent body fat, tumor necrosis factor- (TNF-), malondialdehyde (MDA), nuclear factor kappa-B (NF-B), matrix metalloprotease-9 (MMP-9), cyclooxygenase-2 (COX-2), and mRNA endothelial nitric oxide synthase (eNOS).
The HFD group had increased body weight, increased systolic and diastolic blood pressure, liver total cholesterol, triglyceride, NF-B, COX-2 and MMP-9 protein levels, and decreased mRNA eNOS in the aorta. Mice of the HFD group receiving RBE had reduced diastolic blood pressure, as well as significantly decreased liver and serum TNF- and MDA levels in the liver, and reduced NF-B levels in both the liver and heart.
These results demonstrate that RBE decreases diastolic blood pressure, the liver lipid droplet accumulation, liver and myocardial NF-B, myocardial COX-2 and MMP-9 protein levels, and oxidative stress. Moreover, RBE may improve endothelial function and may alleviate adverse health effects associated with obesity including obesity-associated hypertension.
炎症和高血压是高脂饮食所致肥胖相关不良反应的主要机制。本研究旨在评估米糠提取物（RBE）对高脂饮食（HFD）诱导的肥胖小鼠动脉血压、肝脂肪变性、炎症和氧化应激的影响。
将雄性ICR小鼠分为四组，包括正常饮食对照组、高脂饮食（60%千卡热量来自脂肪）组、接受RBE（220毫克/千克/天）治疗的高脂饮食组和接受1100毫克/千克/天治疗的高脂饮食组，为期八周。除体重和动脉血压外，我们还测定了肝脏总胆固醇、甘油三酯值，以及体脂百分比、肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）、核因子κB（NF-κB）、基质金属蛋白酶-9（MMP-9）、环氧合酶-2（COX-2）和mRNA内皮型一氧化氮合酶（eNOS）。
高脂饮食组体重增加、收缩压和舒张压升高、肝脏总胆固醇、甘油三酯、NF-κB、COX-2和MMP-9蛋白水平升高，主动脉中mRNA eNOS降低。接受RBE的高脂饮食组小鼠舒张压降低，肝脏和血清中TNF-α和肝脏中MDA水平显著降低，肝脏和心脏中NF-κB水平降低。
这些结果表明，RBE可降低舒张压、肝脏脂质滴积累、肝脏和心肌NF-κB、心肌COX-2和MMP-9蛋白水平以及氧化应激。此外，RBE可能改善内皮功能，并可能减轻与肥胖相关的不良健康影响，包括肥胖相关高血压。
