Wang Canhong, Peng Deqian, Liu Yangyang, Yu Zhangxin, Guo Peng, Wei Jianhe
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, China.
School of Pharmacy, Hainan Medical College, Haikou, Hainan 571199, China.
Cardiol Res Pract. 2020 Jul 9;2020:3640815. doi: 10.1155/2020/3640815. eCollection 2020.
Agarwood is a traditional medicine used for treating some diseases, including painful and ischemic diseases. This study was carried out to investigate the potential cardioprotective effect of the whole-tree agarwood-inducing technique-produced agarwood alcohol extract (WTAAE) on isoproterenol- (ISO-) induced myocardial ischemia (MI) in rats and explore the underlying molecular mechanisms. Compared to the MI group, WTAAE pretreatment significantly improved ST wave abnormal-elevation, mitigated myocardial histological damage; decreased creatinine kinase (CK), lactate dehydrogenase (LDH), alanine transaminase (ALT), and aspartate transaminase (AST) levels; reduced hydrogen peroxide (HO) and lipid peroxide (LPO) production; and increased total antioxidant capacity (T-AOC) and catalase (CAT) activities. Moreover, agarwood alcohol extracts (AAEs) markedly enhanced the mRNA levels of Nrf2-ARE pathway, and Bcl-2 reduced the apoptotic Bax family mRNA expressions. In addition, the effect of WTAAE was greater than that of wild agarwood alcohol extract (WAAE) and burning-chisel-drilling agarwood alcohol extract (FBAAE). All of these data indicate that WTAAE exerted the protective effects of MI, and its mechanism was associated with upregulating Nrf2-ARE and suppressing Bcl-2 pathways.
沉香是一种用于治疗某些疾病的传统药物，包括疼痛性疾病和缺血性疾病。本研究旨在探讨全树结香技术制备的沉香醇提取物（WTAAE）对异丙肾上腺素（ISO）诱导的大鼠心肌缺血（MI）的潜在心脏保护作用，并探索其潜在的分子机制。与MI组相比，WTAAE预处理显著改善ST段异常抬高，减轻心肌组织学损伤；降低肌酸激酶（CK）、乳酸脱氢酶（LDH）、丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）水平；减少过氧化氢（HO）和脂质过氧化物（LPO）的产生；并增加总抗氧化能力（T-AOC）和过氧化氢酶（CAT）活性。此外，沉香醇提取物（AAEs）显著提高Nrf2-ARE途径的mRNA水平，而Bcl-2降低凋亡Bax家族mRNA表达。此外，WTAAE的作用大于野生沉香醇提取物（WAAE）和燃凿钻法沉香醇提取物（FBAAE）。所有这些数据表明WTAAE对MI具有保护作用，其机制与上调Nrf2-ARE和抑制Bcl-2途径有关。
