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通过发光二极管（LED）光照提高富含具有治疗作用的植物化学物质的杂交兰花类原球茎（PLB）中次生代谢产物的产量。

Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of hybrid orchid rich in phytochemicals with therapeutic effects.

作者信息

Yeow Lit Chow, Chew Bee Lynn, Sreeramanan Subramaniam

机构信息

School of Biological Sciences, Universiti Sains Malaysia, 11700 Gelugor, Pulau Pinang, Malaysia.

Centre for Chemical Biology, Universiti Sains Malaysia, 11900 Bayan Lepas, Penang, Malaysia.

出版信息

Biotechnol Rep (Amst). 2020 Jul 2;27:e00497. doi: 10.1016/j.btre.2020.e00497. eCollection 2020 Sep.

DOI:10.1016/j.btre.2020.e00497
PMID:32695616
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7365977/
Abstract

Gas-chromatography-mass-spectrometry revealed the presence of various bioactive compounds with anticancer properties in protocorm-like-body (PLB) cultures of a hybrid orchid ( Enopi x Pink Lady). Pre-illumination of red fluorescent light lessened the stimulating effects of light-emitting diodes (LEDs) on secondary metabolites production among PLB cultures, possibly due to habituation. The highest flavonoid content of 16.79 μmol/ g of fresh weight (FW) was achieved under blue-red (1:1) LED for PLBs pre-treated with white LED for more than 3 subculture cycles. Phenolics content significantly reduced as PLBs pre-cultured under red fluorescent light for 2 subculture cycles were exposed to LED illuminations, where far red LED resulted in the lowest total phenolic content (18.85 μmol/ g FW). High intensity green LED (16.9 μmol/s) enhanced the accumulation of phenolics while amino acids such as L-leucine, glycine and proline exhibited no significant stimulating effect for secondary metabolites production.

摘要

气相色谱 - 质谱分析显示，在一种杂交兰花（Enopi x Pink Lady）的原球茎样体（PLB）培养物中存在多种具有抗癌特性的生物活性化合物。红色荧光预照射减轻了发光二极管（LED）对PLB培养物中次生代谢产物产生的刺激作用，这可能是由于适应性所致。对于经过超过3次继代培养周期的白色LED预处理的PLB，在红蓝（1:1）LED光照下，黄酮类化合物含量最高，达到16.79 μmol/g鲜重（FW）。当在红色荧光下预培养2次继代培养周期的PLB暴露于LED光照时，酚类物质含量显著降低，其中远红光LED导致总酚含量最低（18.85 μmol/g FW）。高强度绿色LED（16.9 μmol/s）促进了酚类物质的积累，而L - 亮氨酸、甘氨酸和脯氨酸等氨基酸对次生代谢产物的产生没有显著的刺激作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/d310cde89a57/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/17f29d4d9186/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/f5d7663957be/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/725a878d95c4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/7e7ac46f507c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/6fb64f80fdea/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/04a131fa48ce/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/d310cde89a57/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/17f29d4d9186/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/f5d7663957be/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/725a878d95c4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/7e7ac46f507c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/6fb64f80fdea/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/04a131fa48ce/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baa9/7365977/d310cde89a57/gr7.jpg

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