Weisbart R H, Mickey M R
J Immunol Methods. 1977;16(3):269-81. doi: 10.1016/0022-1759(77)90204-6.
A reliable microassay for human leukocyte migration is described by which 50 to 100 assays can be performed each day in quadruplicate with the number of indicator cells obtainable from 10 to 20 ml of peripheral blood. The reproducibility of this method is demonstrated with respect to the variability among replicate test wells (including reading), the variability among different test readers, the variability among replicate cultures, and the variability of using indicator cells from different subjects. A microculture system is described that requires only 75,000 mononuclear cells to consistently produce detectable polymorphonuclear leukocyte migration inhibition factor in response to PPD. The reproducibility of this culture system is demonstrated with respect to the variability of lymphocyte responsiveness on repetitive testing in the same individuals.
本文描述了一种可靠的人类白细胞迁移微量测定法，每天可进行50至100次测定，一式四份，所需指示细胞数量可从10至20毫升外周血中获取。该方法的可重复性体现在重复测试孔之间（包括读数）的变异性、不同测试读数器之间的变异性、重复培养物之间的变异性以及使用不同受试者的指示细胞的变异性。本文还描述了一种微量培养系统，该系统仅需75,000个单核细胞就能持续产生可检测到的针对结核菌素纯蛋白衍生物（PPD）的多形核白细胞迁移抑制因子。该培养系统的可重复性体现在同一个体重复测试时淋巴细胞反应性的变异性。
