Wang Jinglei, Hu Haijiao, Wang Wuhong, Wei Qingzhen, Hu Tianhua, Bao Chonglai
Institute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Plants (Basel). 2020 Jul 20;9(7):915. doi: 10.3390/plants9070915.
Plant heat shock factors (Hsfs) play crucial roles in various environmental stress responses. Eggplant ( L.) is an agronomically important and thermophilic vegetable grown worldwide. Although the functions of Hsfs under environmental stress conditions have been characterized in the model plant and tomato, their roles in responding to various stresses remain unclear in eggplant. Therefore, we characterized the eggplant SmeHsf family and surveyed expression profiles mediated by the SmeHsfs under various stress conditions. Here, using reported Hsfs from other species as queries to search SmeHsfs in the eggplant genome and confirming the typical conserved domains, we identified 20 SmeHsf genes. The SmeHsfs were further classified into 14 subgroups on the basis of their structure. Additionally, quantitative real-time PCR revealed that SmeHsfs responded to four stresses-cold, heat, salinity and drought-which indicated that SmeHsfs play crucial roles in improving tolerance to various abiotic stresses. The expression pattern of exhibited the most immediate response to the various environmental stresses, except drought. The genome-wide identification and abiotic stress-responsive expression pattern analysis provide clues for further analysis of the roles and regulatory mechanism of SmeHsfs under environmental stresses.
植物热激因子（Hsfs）在各种环境胁迫响应中发挥着关键作用。茄子（L.）是一种在全球范围内种植的具有重要农艺价值的喜温蔬菜。尽管在模式植物和番茄中已经对环境胁迫条件下Hsfs的功能进行了表征，但它们在茄子应对各种胁迫中的作用仍不清楚。因此，我们对茄子SmeHsf家族进行了表征，并研究了SmeHsfs在各种胁迫条件下介导的表达谱。在这里，我们以其他物种中已报道的Hsfs为查询序列，在茄子基因组中搜索SmeHsfs，并确认典型的保守结构域，从而鉴定出20个SmeHsf基因。根据其结构，SmeHsfs进一步分为14个亚组。此外，实时定量PCR显示SmeHsfs对冷、热、盐度和干旱四种胁迫有响应，这表明SmeHsfs在提高对各种非生物胁迫的耐受性中发挥着关键作用。除干旱外， 的表达模式对各种环境胁迫表现出最迅速的响应。全基因组鉴定和非生物胁迫响应表达模式分析为进一步分析SmeHsfs在环境胁迫下的作用和调控机制提供了线索。
