Guangdong Eye Institute, Department of Ophthalmology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences/The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.
Aier Institute of Refractive Surgery, Refractive Surgery Center, Guangzhou Aier Eye Hospital, Guangzhou, China.
J Diabetes Res. 2020 Jun 30;2020:4631290. doi: 10.1155/2020/4631290. eCollection 2020.
To analyze the levels of B-cell-produced antibodies in the vitreous humor of patients with or without diabetic retinopathy (DR) both qualitatively and quantitatively.
A total of 52 type 2 diabetes mellitus (T2DM) with DR patients and 52 control subjects without diabetes mellitus or inflammatory diseases were included in this prospective study. The levels of immunoglobulin (Ig)A, IgM, and IgG subtypes were measured using a magnetic color-bead-based multiplex assay.
The concentrations of IgA, IgM, and total antibodies in the DR group were significantly higher than those in the control group (all < 0.001), but there was no significant difference in the 4 IgG subtypes between the two groups after Bonferroni correction. Pearson's correlation analysis revealed low negative correlations between levels of antibodies (IgA, IgM) and estimated glomerular filtration rate (eGFR, = -0.443, = -0.377, respectively, both < 0.05). Furthermore, multiple linear regression analysis yielded three equations to predict the concentrations of IgA, IgM, and total antibodies in the vitreous humor according to eGFR and other clinical variables ( = 0.542, = 0.461, and = 0.312, respectively, all < 0.05).
Increased levels of IgA, IgM, and total antibodies produced by B cells were observed in the vitreous humor of T2DM patients with DR. There were low negative correlations between levels of antibodies (IgA, IgM) and eGFR.
定性和定量分析糖尿病视网膜病变（DR）患者和无糖尿病或炎症性疾病患者玻璃体中 B 细胞产生的抗体水平。
本前瞻性研究纳入了 52 例 2 型糖尿病（T2DM）伴 DR 患者和 52 例无糖尿病或炎症性疾病对照者。采用基于磁性彩色珠的多重分析测定免疫球蛋白（Ig）A、IgM 和 IgG 亚型的水平。
DR 组的 IgA、IgM 和总抗体浓度明显高于对照组（均<0.001），但经 Bonferroni 校正后，两组间 4 种 IgG 亚型无显著差异。Pearson 相关分析显示，抗体（IgA、IgM）水平与估算肾小球滤过率（eGFR）之间呈低度负相关（分别为=-0.443、=-0.377，均<0.05）。此外，多元线性回归分析得出三个方程，根据 eGFR 和其他临床变量预测玻璃体中 IgA、IgM 和总抗体的浓度（分别为=0.542、=0.461 和=0.312，均<0.05）。
T2DM 伴 DR 患者玻璃体中观察到 B 细胞产生的 IgA、IgM 和总抗体水平升高。抗体（IgA、IgM）水平与 eGFR 之间呈低度负相关。
