Institute of Physiology and Biochemistry "Ivan Djaja", Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia.
Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia.
Molecules. 2020 Jul 23;25(15):3343. doi: 10.3390/molecules25153343.
Recently, there has been an increasing interest in the chemistry and biological potential of mosses, since a large number of biologically active compounds have been found within these species. This study aimed at examining the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory/antineurodegenerative, and antitumor activities) of moss Hedw. extracts. Corresponding extracts have been obtained applying Soxhlet extractor. The chemical characterization was performed using spectrophotometric assays and liquid chromatography-mass spectrometry (LC-MS). The extracts were analyzed for antioxidant activity and for inhibitory activities on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase. Additionally, extracts were tested against four cell lines-MRC-5, BV2, HCT-116, and MDA-MB-231-for antitumor and anti-inflammatory activities. Chemical analysis of extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. Major compounds identified by LC-MS in were kaempferol and five phenolic acids: -hydroxybenzoic, protocatechuic, -coumaric, gallic, and caffeic acid. According to biochemical assays the investigated extracts exhibited significant immunomodulatory potential. Significant antiproliferative potential against MDA-MB-231 cells has been observed together with the promising anti-neuroinflammatory application. The obtained data suggest that moss is a valuable natural source of biologically active compounds with potential application in the pharmaceutical industry.
最近，人们对苔藓的化学和生物潜力越来越感兴趣，因为在这些物种中发现了大量具有生物活性的化合物。本研究旨在研究苔藓 Hedw. 提取物的化学成分和免疫调节潜力（抗氧化、抗糖尿病、抗神经炎症/神经退行性、抗肿瘤活性）。应用索氏提取器获得相应的提取物。采用分光光度法和液相色谱-质谱联用（LC-MS）进行化学表征。分析提取物的抗氧化活性以及对α-葡萄糖苷酶、α-淀粉酶、乙酰胆碱酯酶和酪氨酸酶的抑制活性。此外，还针对四种细胞系-MRC-5、BV2、HCT-116 和 MDA-MB-231-测试提取物的抗肿瘤和抗炎活性。提取物的化学分析表明存在类黄酮、酚酸和三萜。通过 LC-MS 在 中鉴定出的主要化合物是山奈酚和五种酚酸：-羟基苯甲酸、原儿茶酸、-香豆酸、没食子酸和咖啡酸。根据生化测定，所研究的提取物表现出显著的免疫调节潜力。对 MDA-MB-231 细胞表现出显著的增殖抑制潜力，并具有有前途的抗神经炎症应用。获得的数据表明，苔藓 是具有生物活性化合物的有价值的天然来源，具有在制药行业应用的潜力。
