Lunić Tanja M, Mandić Marija R, Oalđe Pavlović Mariana M, Sabovljević Aneta D, Sabovljević Marko S, Božić Nedeljković Biljana Đ, Božić Bojan Đ
Institute of Physiology and Biochemistry "Ivan Đaja", Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia.
Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia.
Plants (Basel). 2022 Jan 1;11(1):123. doi: 10.3390/plants11010123.
Numerous representatives of mosses, including , have been used to alleviate different inflammation-related conditions. However, the mode of action underlying this anti-inflammatory potential has been poorly understood. Moreover, the influence of seasonality on the chemical composition and biological activity of mosses is generally overlooked. This study aimed to investigate the influence of seasonal changes (spring, summer, and autumn) on secondary metabolite composition and biological activities of ethyl acetate extracts. Antioxidant activity was measured using β-carotene bleaching assay, while MTT, NBT, ELISA, and Griess assays were carried out to explore the anti-neuroinflammatory and neuroprotective potential of extracts. Inhibitory activities on acetylcholinesterase and tyrosinase were assessed experimentally and by docking analysis. The highest content of secondary metabolites and antioxidant activity were observed in moss during the summer. Extracts inhibited the secretion of ROS, NO, TNF-α, and IL-6, alleviating the inflammatory potential of HO and LPS in microglial and neuronal cells. Strong inhibitory effects on acetylcholinesterase and tyrosinase were observed in vitro. Docking analyses revealed high-affinity interactions of secondary metabolites present in with important enzyme residues. Altogether, these results reveal the neuroprotective potential and the significance of seasonal fluctuations on secondary metabolite content and biological activities in moss .
包括[具体种类未给出]在内的众多苔藓植物已被用于缓解各种与炎症相关的病症。然而，这种抗炎潜力背后的作用模式却鲜为人知。此外，季节性对苔藓植物化学成分和生物活性的影响通常被忽视。本研究旨在探讨季节变化（春季、夏季和秋季）对苔藓乙酸乙酯提取物次生代谢产物组成和生物活性的影响。使用β - 胡萝卜素漂白法测定抗氧化活性，同时进行MTT、NBT、ELISA和Griess试验以探究提取物的抗神经炎症和神经保护潜力。通过实验和对接分析评估对乙酰胆碱酯酶和酪氨酸酶的抑制活性。在夏季苔藓中观察到次生代谢产物含量和抗氧化活性最高。提取物抑制了活性氧（ROS）、一氧化氮（NO）、肿瘤坏死因子 -α（TNF -α）和白细胞介素 -6（IL -6）的分泌，减轻了小胶质细胞和神经元细胞中脂多糖（LPS）和脂多糖（HO）的炎症潜力。在体外观察到对乙酰胆碱酯酶和酪氨酸酶有强烈的抑制作用。对接分析揭示了[具体苔藓种类]中存在的次生代谢产物与重要酶残基之间的高亲和力相互作用。总之，这些结果揭示了苔藓的神经保护潜力以及季节性波动对苔藓次生代谢产物含量和生物活性的重要性。
