着丝粒核心蛋白 A 核小体——染色质中着丝粒的嵌入基序：结构生物学的启示。
CENP-A nucleosome-a chromatin-embedded pedestal for the centromere: lessons learned from structural biology.
机构信息
Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Faculty of Medicine, University of Oslo, Oslo 0318, Norway.
Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern NO-0315, Norway.
出版信息
Essays Biochem. 2020 Sep 4;64(2):205-221. doi: 10.1042/EBC20190074.
The centromere is a chromosome locus that directs equal segregation of chromosomes during cell division. A nucleosome containing the histone H3 variant CENP-A epigenetically defines the centromere. Here, we summarize findings from recent structural biology studies, including several CryoEM structures, that contributed to elucidate specific features of the CENP-A nucleosome and molecular determinants of its interactions with CENP-C and CENP-N, the only two centromere proteins that directly bind to it. Based on those findings, we propose a role of the CENP-A nucleosome in the organization of centromeric chromatin beyond binding centromeric proteins.
着丝粒是染色体上的一个基因座，它在细胞分裂过程中指导染色体的均等分离。一个含有组蛋白 H3 变体 CENP-A 的核小体在表观遗传上定义了着丝粒。在这里，我们总结了最近结构生物学研究的发现，包括几个 CryoEM 结构，这些发现有助于阐明 CENP-A 核小体的特定特征及其与 CENP-C 和 CENP-N 的相互作用的分子决定因素，CENP-C 和 CENP-N 是仅有的两个直接与其结合的着丝粒蛋白。基于这些发现，我们提出 CENP-A 核小体在结合着丝粒蛋白之外，在着丝粒染色质的组织中的作用。
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