Marine Biotechnology Department, Stazione Zoologica Anton Dohrn, Villa Comunale, CAP80121 Napoli, Italy.
Faculty of Medicine and Health Sciences, Linköping University, 58183 Linköping, Sweden.
Mar Drugs. 2020 Jul 24;18(8):386. doi: 10.3390/md18080386.
Many dinoflagellates species, especially of the genus, produce a series of toxins with tremendous impacts on human and environmental health, and tourism economies. was discovered for the first time in the Gulf of Naples, and it is not known to produce saxitoxins. However, a clone of from the same Gulf showed copepod reproduction impairment and antiproliferative activity. In this study, the full transcriptome of the dinoflagellate is presented in both control and phosphate starvation conditions. RNA-seq approach was used for in silico identification of transcripts that can be involved in the synthesis of toxic compounds. Phosphate starvation was selected because it is known to induce toxin production for other spp. Results showed the presence of three transcripts related to saxitoxin synthesis (sxtA, sxtG and sxtU), and others potentially related to the synthesis of additional toxic compounds (e.g., 44 transcripts annotated as "polyketide synthase"). These data suggest that even if this clone does not produce saxitoxins, it has the potential to produce toxic metabolites, in line with the previously observed activity. These data give new insights into toxic microalgae, toxin production and their potential applications for the treatment of human pathologies.
许多甲藻物种，尤其是属，产生一系列毒素，对人类健康、环境健康和旅游经济都有巨大影响。最早在那不勒斯湾发现了，它不产生石房蛤毒素。然而，来自同一海湾的一个克隆显示桡足类动物繁殖受损和抗增殖活性。在这项研究中，展示了甲藻的完整转录组在对照和磷酸盐饥饿条件下。使用 RNA-seq 方法对可能参与合成有毒化合物的转录本进行了计算机识别。选择磷酸盐饥饿是因为它已知会诱导其他 spp 产生毒素。结果表明存在三个与石房蛤毒素合成相关的转录本（sxtA、sxtG 和 sxtU），以及其他可能与其他有毒化合物合成相关的转录本（例如，44 个注释为“聚酮合酶”的转录本）。这些数据表明，即使这个克隆不产生石房蛤毒素，它也有可能产生有毒代谢物，这与之前观察到的活性一致。这些数据为有毒微藻、毒素产生及其在治疗人类疾病方面的潜在应用提供了新的见解。
