Biotechnology Center (BIOTEC), TU Dresden, 01307, Dresden, Germany.
PharmAI GmbH, Tatzberg 47, 01307, Dresden, Germany.
Sci Rep. 2020 Jul 28;10(1):12647. doi: 10.1038/s41598-020-69100-0.
Storage and directed transfer of information is the key requirement for the development of life. Yet any information stored on our genes is useless without its correct interpretation. The genetic code defines the rule set to decode this information. Aminoacyl-tRNA synthetases are at the heart of this process. We extensively characterize how these enzymes distinguish all natural amino acids based on the computational analysis of crystallographic structure data. The results of this meta-analysis show that the correct read-out of genetic information is a delicate interplay between the composition of the binding site, non-covalent interactions, error correction mechanisms, and steric effects.
信息的存储和定向传递是生命发展的关键要求。然而，如果我们的基因上存储的信息不能正确解读，那么这些信息将毫无用处。遗传密码定义了解码这些信息的规则集。氨酰-tRNA 合成酶是这个过程的核心。我们通过对晶体结构数据的计算分析，广泛地描述了这些酶如何根据氨基酸的组成来区分所有天然氨基酸。这项荟萃分析的结果表明，遗传信息的正确读取是结合部位的组成、非共价相互作用、纠错机制和空间效应之间的微妙相互作用。
