Qian Bingjun, Zhao Xin, Yang Ye, Tian Chongchong
Institute of Biomedical Technology Jiangsu Vocational College of Medicine Yancheng China.
Department of Food Science and Engineering Shanghai Jiao Tong University Shanghai China.
Food Sci Nutr. 2020 Jun 13;8(7):3947-3956. doi: 10.1002/fsn3.1710. eCollection 2020 Jul.
Recent studies have confirmed that the peptide fractions derived from marine organisms exhibit good antioxidant and anti-inflammatory activity, and oyster is an excellent nutrient resource with high-protein content. In this study, the peptide fractions from oyster soft tissue were prepared after hydrolysis by pepsin (pH 2, 37°C), trypsin (pH 8, 37°C), and Maxipro PSP (pH 4.2, 50°C) with the optimized parameters (enzyme-to-substrate (E/S) ratio, 1:100 (w/w); hydrolysis time, 4 hr), respectively. Four fractions named as PEP-1, PEP-2, TRYP-2, and MIX-2 were obtained after separation with elution consisting of 20% or 40% ethanol. The MIX-2 exhibited the highest hydrophobicity correlated well with its hydrophobic amino acid content, and TRYP-2 exhibited much better antioxidant activity than other three elution samples. Furthermore, all of the bioactive peptide fractions were noncytotoxic and could selectively repress pro-inflammatory mediators, TNF-α, IL-1β, IL-6, and i-NOS, at transcription level in RAW264.7 macrophage cells after LPS stimulation. The result suggests that the peptide fraction TRYP-2 from oyster soft tissue hydrolysates might be a potential resource for natural anti-inflammatory components.
最近的研究证实，源自海洋生物的肽组分具有良好的抗氧化和抗炎活性，而牡蛎是一种高蛋白含量的优质营养资源。在本研究中，分别采用胃蛋白酶（pH 2，37°C）、胰蛋白酶（pH 8，37°C）和Maxipro PSP（pH 4.2，50°C）在优化参数（酶与底物（E/S）比例为1:100（w/w）；水解时间为4小时）下水解制备牡蛎软组织的肽组分。用20%或40%乙醇洗脱分离后，得到了四个组分，分别命名为PEP-1、PEP-2、TRYP-2和MIX-2。MIX-2表现出最高的疏水性，这与其疏水氨基酸含量密切相关，并且TRYP-2表现出比其他三个洗脱样品更好的抗氧化活性。此外，所有生物活性肽组分均无细胞毒性，并且在LPS刺激后，能够在RAW264.7巨噬细胞中转录水平选择性抑制促炎介质TNF-α、IL-1β、IL-6和诱导型一氧化氮合酶（i-NOS）。结果表明，牡蛎软组织水解物中的肽组分TRYP-2可能是天然抗炎成分的潜在来源。
