González Garza Nancy Gisela, Chuc Koyoc Janice Azucena, Torres Castillo Jorge Ariel, García Zambrano Eduardo Alejandro, Betancur Ancona David, Chel Guerrero Luis, Sinagawa García Sugey Ramona
Laboratorio de Biotecnología, Facultad de Agronomía, Universidad Autónoma de Nuevo León, Francisco Villa S/N Col, Ex hacienda El Canadá, C.P. 66050 General Escobedo, N.L. Mexico.
Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Periférico Norte Km. 33.5, Tablaje Catastral 13615, Colonia Chuburná de Hidalgo Inn, 97203 Mérida, Yucatán Mexico.
J Food Sci Technol. 2017 Dec;54(13):4268-4276. doi: 10.1007/s13197-017-2898-8. Epub 2017 Oct 29.
(Moringaceae) is a specie of significant importance because of its multiple nutraceutical properties, that has led to increase in its consumption. The seeds contain a high percentage of protein (37.48%). However, little is known about the bioactive properties of these proteins and peptides, especially those generated by enzymatic hydrolysis. The objective of this study was to evaluate the biofunctional properties of total hydrolysates (TH) and peptide fractions from protein isolates of moringa seeds. Isoelectric protein isolates were prepared and TH were obtained by digestion with trypsin, chymotrypsin and pepsin-trypsin for 2.5 and 5 h. TH were fractioned by ultrafiltration (UF) with a 10 kDa membrane to generate the peptide fractions. In all treatments, the antioxidant capacity was significantly higher in peptide fractions > 10 kDa with 5 h of hydrolysis. The results showed that the fraction > 10 kDa of pepsin-trypsin digested for 5 h presented a better Angiotensin Converting Enzyme inhibition (ACE-I) activity with an IC of 0.224 μg/μl. Also, antidiabetic activity was enhanced in pepsin-trypsin treatment with 5 h of hydrolysis showing an IC of 0.123 μg/μl. Finally, this study showed that hydrolysates of moringa seed proteins had excellent in vitro nutraceutical potential.
辣木科是一种具有重要意义的物种，因其具有多种营养保健特性，导致其消费量增加。种子含有高比例的蛋白质（37.48%）。然而，对于这些蛋白质和肽的生物活性特性，尤其是酶促水解产生的那些，人们了解甚少。本研究的目的是评估辣木种子蛋白质分离物的总水解产物（TH）和肽组分的生物功能特性。制备了等电蛋白质分离物，并通过用胰蛋白酶、胰凝乳蛋白酶和胃蛋白酶 - 胰蛋白酶消化2.5小时和5小时获得TH。通过用10 kDa膜进行超滤（UF）对TH进行分级，以产生肽组分。在所有处理中，水解5小时的>10 kDa肽组分的抗氧化能力显著更高。结果表明，胃蛋白酶 - 胰蛋白酶消化5小时的>10 kDa组分表现出更好的血管紧张素转换酶抑制（ACE - I）活性，IC为0.224μg/μl。此外，水解5小时的胃蛋白酶 - 胰蛋白酶处理增强了抗糖尿病活性，IC为0.123μg/μl。最后，本研究表明辣木种子蛋白水解产物具有优异的体外营养保健潜力。
