Department of Microbiology and Immunology, University of Iowa Carver College of Medicine, Iowa City, Iowa
Hennepin Life Sciences, Minneapolis, Minnesota, USA.
mSphere. 2020 Jul 29;5(4):e00552-20. doi: 10.1128/mSphere.00552-20.
is a highly significant infection problem in health care centers, particularly after surgery. It has been shown that nearly 80% of infections following surgery are the same as those in the anterior nares of patients, suggesting that the anterior nares is the source of the infection strain. This has led to the use of mupirocin ointment being applied nasally to reduce infections; mupirocin resistance is being observed. This study was undertaken to determine whether gel composed of 5% glycerol monolaurate solubilized in a glycol-based, nonaqueous gel (5% GML gel) could be used as an alternative. In our study, 40 healthy human volunteers swabbed their anterior nares for 3 days with the 5% GML gel. Prior to swabbing and 8 to 12 h after swabbing, and coagulase-negative staphylococcal CFU per milliliter were determined by plating the swabs on mannitol salt agar. Fourteen of the volunteers had in their nares prior to 5% GML gel treatment, most persons with the organisms present in both nares; five had pure cultures of All participants without pure culture of were cocolonized with and coagulase-negative staphylococci. Five of the strains produced the superantigens commonly associated with toxic shock syndrome, though none of the participants became ill. For both and coagulase-negative staphylococci, the 5% GML gel treatment resulted in a 3-log-unit reduction in microorganisms. For , the reduction persisted for 2 or 3 days. In this microflora study, we show that a 5% glycerol monolaurate nonaqueous gel is safe for use in the anterior nares. The gel was effective in reducing nasally, a highly significant hospital-associated pathogen. The gel may be a useful alternative or additive to mupirocin ointment for nasal use prior to surgery, noting that 80% of hospital-associated infections are due to the same organism found in the nose. This gel also kills all enveloped viruses tested and should be considered for studies to reduce infection and transmission of coronaviruses and influenza viruses.
是医疗机构中一个非常严重的感染问题，尤其是在手术后。研究表明，近 80%的术后感染与患者前鼻孔中的感染相同，这表明前鼻孔是感染株的来源。这导致人们使用莫匹罗星软膏进行鼻腔给药以减少感染；但现在已经观察到莫匹罗星耐药的情况。本研究旨在确定是否可以使用 5%甘油单月桂酸酯溶于基于二醇的非水凝胶（5% GML 凝胶）组成的凝胶作为替代物。在我们的研究中，40 名健康志愿者连续 3 天用 5% GML 凝胶擦拭他们的前鼻孔。在擦拭前和擦拭后 8 至 12 小时，通过将拭子接种在甘露醇盐琼脂上来确定每毫升的 和凝固酶阴性葡萄球菌 CFU。在 5% GML 凝胶治疗之前，有 14 名志愿者的鼻腔中存在 ，大多数人在两个鼻腔中都存在该生物体；5 人有 的纯培养物；所有参与者均与 和凝固酶阴性葡萄球菌共同定植。有 5 株 的菌株产生与中毒性休克综合征相关的超抗原，尽管没有参与者生病。对于 和凝固酶阴性葡萄球菌，5% GML 凝胶治疗导致微生物减少 3 个对数单位。对于 ，减少持续了 2 或 3 天。在这项微生物群研究中，我们表明 5%甘油单月桂酸酯非水凝胶可安全用于前鼻孔。该凝胶可有效减少鼻腔内的定植，这是一种高度重要的医院相关病原体。该凝胶可作为手术前鼻腔使用莫匹罗星软膏的替代物或添加剂，因为 80%的医院相关 感染是由鼻腔中发现的相同生物体引起的。这种凝胶还可以杀死所有测试的包膜病毒，因此应该考虑将其用于减少冠状病毒和流感病毒的感染和传播的研究。
