Fernández-Poyatos María Del Pilar, Zengin Gökhan, Salazar-Mendías Carlos, Ruiz-Medina Antonio, Sinan Kouadio Ibrahime, Llorent-Martínez Eulogio J
Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas, Jaén E-23071, Spain.
Department of Biology, Science Faculty, Selcuk University, Campus, Konya, Turkey.
J Anal Methods Chem. 2020 Jul 11;2020:8885169. doi: 10.1155/2020/8885169. eCollection 2020.
In this work, we report the phenolic composition and bioactivity of the aerial parts of three species of (, , and ) to study their potential as sources of bioactive compounds to revalorize them and contribute to the conservation of these plant species. Samples were collected in different locations in the province of Jaén (southeast of Spain), and qualitative and quantitative analyses of phenolic compounds were performed by high-performance liquid chromatography with diode array and mass spectrometry detection. presented the highest concentration of phenolic compounds (58 mg/g DE). The most abundant compound in and was rutin (35 mg/g DE and 11.7 mg/g DE, respectively), whereas isorhamnetin-O-rutinoside was dominant in (11.5 mg/g DE). Several assays were performed to evaluate the potential bioactivity of the three species of . These assays included antioxidant and radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating, and enzyme inhibitory activity (acetylcholinesterase, amylase, butyrylcholinesterase, glucosidase, and tyrosinase). In general, all methanolic extracts presented the highest phenolic and flavonoid contents, as well as the highest radical scavenging, antioxidant, and enzyme inhibitory properties. This relationship between phenolics and bioactivity was confirmed by multivariate analysis.
在本研究中，我们报告了三种[植物名称]地上部分的酚类成分和生物活性，以研究它们作为生物活性化合物来源的潜力，从而提升其价值并有助于保护这些植物物种。样品采集于哈恩省（西班牙东南部）的不同地点，通过高效液相色谱结合二极管阵列和质谱检测对酚类化合物进行了定性和定量分析。[植物名称1]的酚类化合物浓度最高（58毫克/克干提取物）。[植物名称2]和[植物名称3]中最丰富的化合物是芦丁（分别为35毫克/克干提取物和11.7毫克/克干提取物），而异鼠李素 - O - 芸香糖苷在[植物名称1]中占主导地位（11.5毫克/克干提取物）。进行了多项试验以评估这三种[植物名称]的潜在生物活性。这些试验包括抗氧化和自由基清除（ABTS和DPPH）、还原能力（CUPRAC和FRAP）、磷钼酸和金属螯合以及酶抑制活性（乙酰胆碱酯酶、淀粉酶、丁酰胆碱酯酶、葡萄糖苷酶和酪氨酸酶）。总体而言，所有甲醇提取物的酚类和黄酮类含量最高，以及自由基清除、抗氧化和酶抑制性能最强。酚类与生物活性之间的这种关系通过多变量分析得到了证实。
