Sharma Shashi, Singh Dinesh Kumar, Gurung Yam Bahadur, Shrestha Swoyam Prakash, Pantha Chiranjibi
Nepal Agricultural Research Council, Nepal.
Institute of Agriculture & Animal Science, Nepal.
Vet Anim Sci. 2018 Jul 21;6:56-63. doi: 10.1016/j.vas.2018.07.002. eCollection 2018 Dec.
A study was conducted to identify the immunomodulatory effect of Stinging nettle () and Aloe vera (oe ) in broiler chicken. A total of 240 birds were divided into 4 treatment groups with 4 replications in each group, each replication containing 15 birds. Treatments were comprised of no Nettle and Aloe vera (T); Nettle 5% (T), Aloe vera @5 ml/lit drinking water (T) and Nettle 5% and Aloe vera @5 ml/lit drinking water (T). The study was conducted under a randomized design. The immunomodulating effect was evaluated by measuring antibody titer against NDV vaccine; and by estimating the albumin, globulin and total protein upto 40 days of age. The effect on liver was estimated by measuring liver enzymes alanine aminotransferase (ALT). The hypoglycemic effect was estimated by measuring glucose level in the serum, and morphological changes in the spleen and bursa were estimated by measuring the ratio score of the respective organs to the body weight. Throughout the experiment, the value for HI titer, albumin, globulin, total protein, spleen weight ratio score, bursa weight ratio score, liver function test and blood glucose of all treatment group were non significant ( > 0.05) except for albumin on 10th day, globulin on 0th day, total protein on 0th day and ratio score of spleen were found to be significant ( ≤ 0.05) at 30 days in all treatment compared to control group. Histopathology of spleen and bursa appeared normal in all treatment and control groups. Result thus suggests that nettle and Aloe vera have non-significant immunomodulatory effects.
进行了一项研究，以确定荨麻和芦荟对肉鸡的免疫调节作用。总共240只鸡被分为4个处理组，每组4个重复，每个重复包含15只鸡。处理包括不添加荨麻和芦荟（T1）；5%荨麻（T2），每升饮用水添加5毫升芦荟（T3）以及5%荨麻和每升饮用水添加5毫升芦荟（T4）。该研究采用随机设计进行。通过测量针对新城疫病毒（NDV）疫苗的抗体滴度来评估免疫调节作用；并在40日龄前估算白蛋白、球蛋白和总蛋白。通过测量肝酶丙氨酸转氨酶（ALT）来评估对肝脏的影响。通过测量血清中的葡萄糖水平来评估降血糖作用，并通过测量各器官与体重的比值分数来评估脾脏和法氏囊的形态变化。在整个实验过程中，所有处理组的血凝抑制（HI）滴度、白蛋白、球蛋白、总蛋白、脾脏重量比值分数、法氏囊重量比值分数、肝功能测试和血糖值均无显著差异（P＞0.05），但与对照组相比，所有处理组在第10天的白蛋白、第0天的球蛋白、第0天的总蛋白以及30天时的脾脏比值分数有显著差异（P≤0.05）。所有处理组和对照组的脾脏和法氏囊组织病理学均正常。因此结果表明，荨麻和芦荟具有不显著的免疫调节作用。
