文献检索，用中文搜 PubMed

INTRODUCTION

Silver nanoparticles (AgNPs) have been shown to promote wound healing and to exhibit antimicrobial properties against a broad range of bacteria. In our previous study, we prepared tannic acid (TA)-modified AgNPs showing a good toxicological profile and immunomodulatory properties useful for potential dermal applications.

METHODS

In this study, in vitro scratch assay, antimicrobial tests, modified lymph node assay as well as a mouse splint wound model were used to access the wound healing potential of TA-modified and unmodified AgNPs.

RESULTS

TA-modified but not unmodified AgNPs exhibited effective antibacterial activity against , and and stimulated migration of keratinocytes in vitro. The tests using the mouse splint wound model showed that TA-modified 33 and 46 nm AgNPs promoted better wound closure, epithelialization, angiogenesis and formation of the granulation tissue. Additionally, AgNPs elicited expression of VEGF-α, PDGF-β and TGF-β1 cytokines involved in wound healing more efficiently in comparison to control and TA-treated wounds. However, both the lymph node assay and the wound model showed that TA-modified AgNPs sized 13 nm can elicit strong inflammatory response not only during wound healing but also when applied to the damaged skin.

CONCLUSION

TA-modified AgNPs sized >26 nm promote wound healing better than TA-modified or unmodified AgNPs. These findings suggest that TA-modified AgNPs sized >26 nm may have a promising application in wound management.

INTRODUCTION

METHODS

RESULTS

CONCLUSION

简介

银纳米粒子（AgNPs）已被证明具有促进伤口愈合的作用，并对广泛的细菌表现出抗菌性能。在我们之前的研究中，我们制备了具有良好毒理学特性和免疫调节特性的单宁酸（TA）修饰的 AgNPs，可用于潜在的皮肤应用。

方法

在这项研究中，我们使用体外划痕试验、抗菌试验、改良淋巴结试验以及小鼠夹板伤口模型来评估 TA 修饰和未修饰的 AgNPs 的伤口愈合潜力。

结果

TA 修饰的 AgNPs 但未修饰的 AgNPs 对、和表现出有效的抗菌活性，并刺激体外角质形成细胞的迁移。使用小鼠夹板伤口模型的试验表明，TA 修饰的 33nm 和 46nm AgNPs 促进了更好的伤口闭合、上皮化、血管生成和肉芽组织形成。此外，与对照和 TA 处理的伤口相比，AgNPs 更有效地诱导了参与伤口愈合的 VEGF-α、PDGF-β 和 TGF-β1 细胞因子的表达。然而，淋巴结试验和伤口模型均表明，13nm 的 TA 修饰 AgNPs 不仅在伤口愈合期间，而且在应用于受损皮肤时，都会引发强烈的炎症反应。