Department of Engineering, Wake Forest University 455 Vine St., Winston-Salem, USA.
Electrophoresis. 2020 Nov;41(21-22):1915-1930. doi: 10.1002/elps.202000034. Epub 2020 Aug 16.
Many cellular functions are affected by and thus can be characterized by a cell's electrophysiology. This has also been found to correspond to other biophysical parameters such as cell morphology and mechanical properties. Dielectrophoresis (DEP) is an electrostatic technique which can be used to examine cellular biophysical parameters through the measuring of single or multiple cell response to electric field induced forces. This label-free method offers many advantages in characterizing a cell population over conventional electrophysiology methods such as patch clamping; however, it has yet to see mainstream pharmacological application. Challenges such as the transdisciplinary nature of the field bridging engineering and the biological sciences, throughput, specificity as well as standardization are being addressed in current literature. This review focuses on the developments of DEP-based cell electrophysiological characterization where determining cellular properties such as membrane conductance and capacitance, and cytoplasmic conductivity are the primary motivation. A brief theoretical review, techniques for obtaining these cell parameters, as well as the resulting cell parameters and their applications are included in this review. This review aims to further support the development of DEP-based cell characterization as an important part of the future of DEP and electrophysiology research.
许多细胞功能受到影响，因此可以通过细胞的电生理学来表征。这也与其他生物物理参数（如细胞形态和机械特性）相对应。介电泳（DEP）是一种静电技术，可通过测量单个或多个细胞对电场诱导力的响应来检测细胞的生物物理参数。与膜片钳等传统电生理学方法相比，这种无标记方法在细胞群体表征方面具有许多优势；然而，它尚未在药理学领域得到广泛应用。当前文献中正在解决该领域的跨学科性质（连接工程学和生物学）、通量、特异性以及标准化等挑战。本综述重点介绍基于 DEP 的细胞电生理学特性分析的发展，其中确定细胞膜电导和电容以及细胞质电导率等细胞特性是主要目的。本文综述包括简要的理论回顾、获取这些细胞参数的技术以及由此产生的细胞参数及其应用。本综述旨在进一步支持基于 DEP 的细胞特性分析的发展，这是 DEP 和电生理学研究未来的重要组成部分。
