Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.
VNUK Institute for Research and Executive Education, The University of Danang, Danang 550 000, Vietnam.
Int J Mol Sci. 2022 Jun 8;23(12):6424. doi: 10.3390/ijms23126424.
Bimetallic nanoparticles are important materials for synthesizing multifunctional nanozymes. A technique for preparing gold-platinum nanoparticles (NPs) on a silica core template (SiO@Au@Pt) using seed-mediated growth is reported in this study. The SiO@Au@Pt exhibits peroxidase-like nanozyme activity has several advantages over gold assembled silica core templates (SiO@Au@Au), such as stability and catalytic performance. The maximum reaction velocity (V) and the Michaelis-Menten constants (K) were and 2.1 × 10 M∙s and 417 µM, respectively. Factors affecting the peroxidase activity, including the quantity of NPs, solution pH, reaction time, and concentration of tetramethyl benzidine, are also investigated in this study. The optimization of SiO@Au@Pt NPs for HO detection obtained in 0.5 mM TMB; using 5 µg SiO@Au@Pt, at pH 4.0 for 15 min incubation. HO can be detected in the dynamic liner range of 1.0 to 100 mM with the detection limit of 1.0 mM. This study presents a novel method for controlling the properties of bimetallic NPs assembled on a silica template and increases the understanding of the activity and potential applications of highly efficient multifunctional NP-based nanozymes.
双金属纳米粒子是合成多功能纳米酶的重要材料。本研究报道了一种在硅核模板（SiO@Au@Pt）上采用种子介导生长制备金-铂纳米粒子（NPs）的方法。SiO@Au@Pt 具有过氧化物酶样纳米酶活性，与金组装的硅核模板（SiO@Au@Au）相比具有稳定性和催化性能等优点。最大反应速度（V）和米氏常数（K）分别为 2.1×10 M∙s 和 417 µM。本研究还考察了影响过氧化物酶活性的因素，包括 NPs 的数量、溶液 pH 值、反应时间和四甲基联苯胺的浓度。在 0.5 mM TMB 中，通过优化 SiO@Au@Pt NPs 用于 HO 检测，获得了最佳条件：使用 5 µg SiO@Au@Pt，在 pH 值为 4.0 下孵育 15 分钟。HO 可以在 1.0 至 100 mM 的动态线性范围内检测到，检测限为 1.0 mM。本研究提出了一种控制双金属 NPs 在硅模板上组装的性质的新方法，增加了对高效多功能基于 NP 的纳米酶的活性和潜在应用的理解。
