Kwon Sangwoo, Yang Woochul, Moon Donggerami, Kim Kyung Sook
Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 130-710, Republic of Korea.
Department of Physics, Dongguk University, Seoul 04620, Republic of Korea.
J Cancer. 2020 Jul 11;11(18):5403-5412. doi: 10.7150/jca.45897. eCollection 2020.
Lower cellular elasticity is a distinguishing feature of cancer cells compared with normal cells. To determine whether cellular elasticity differs based on cancer cell type, cells were selected from three different cancer types including breast, cervix, and lung. For each cancer type, one counterpart normal cell and three types of cancer cells were selected, and their elasticity was measured using atomic force microscopy (AFM). The elasticity of normal cells was in the order of MCF10A > WI-38 ≥ Ect1/E6E7 which corresponds to the counterpart normal breast, lung, and cervical cancer cells, respectively. All cancer cells exhibited lower elasticity than their counterpart normal cells. Compared with the counterpart normal cells, the difference in cellular elasticity was the greatest in cervical cancer cells, followed by lung and breast cancer cells. This result indicates lower elasticity is a unique property of cancer cells; however, the reduction in elasticity may depend on the histological origin of the cells. The F-actin cytoskeleton of cancer cells was different in structure and content from normal cells. The F-actin is mainly distributed at the periphery of cancer cells and its content was mostly lower than that seen in normal cells.
与正常细胞相比，较低的细胞弹性是癌细胞的一个显著特征。为了确定细胞弹性是否因癌细胞类型而异，从包括乳腺癌、宫颈癌和肺癌在内的三种不同癌症类型中选取细胞。对于每种癌症类型，选取一种对应的正常细胞和三种癌细胞，并使用原子力显微镜（AFM）测量它们的弹性。正常细胞的弹性顺序为MCF10A > WI - 38 ≥ Ect1/E6E7，分别对应于乳腺癌、肺癌和宫颈癌的正常细胞。所有癌细胞的弹性均低于其对应的正常细胞。与对应的正常细胞相比，细胞弹性差异在宫颈癌细胞中最大，其次是肺癌和乳腺癌细胞。这一结果表明较低的弹性是癌细胞的独特特性；然而，弹性的降低可能取决于细胞的组织学来源。癌细胞的F - 肌动蛋白细胞骨架在结构和含量上与正常细胞不同。F - 肌动蛋白主要分布在癌细胞的周边，其含量大多低于正常细胞。
