Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
College of Veterinary Medicine, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, Jiangsu 225300, PR China.
Int J Biol Macromol. 2023 Mar 1;230:123386. doi: 10.1016/j.ijbiomac.2023.123386. Epub 2023 Jan 23.
In this experiment, the polysaccharide was extracted from Pueraria lobata (Willd.) Ohwi, and its structural characteristics and bioactivity were investigated. The results showed that Pueraria lobata polysaccharide (PLP) was composed of fucose, arabinose, galactose, glucose, xylose, mannose in a molar proportion of 0.09:1.25:2.19:95.74:0.43:0.30 with a number molar masses (Mn) weight of 14.463 kDa. Besides, FT-IR, Methylation, and NMR analysis revealed that PLP were mainly composed of the main chain →4)-α-Glcp (1→ and →4,6)-α-Glcp (1→, and the branched chain α-Glcp (1→. In vitro experiment, the results showed that PLP could stimulate the expression of surface molecules on RAW264.7 and (T and B) lymphocytes proliferation, simultaneously to stimulate their cytokines secretion. In vivo experiment, the immune organ index, cytokine content, and T lymphocyte subtype in cyclophosphamide-induced immunosuppressed mice could be improved by PLP. These data proved that PLP could be used as a useful immunomodulator to enhance the immune activity of RAW264.7, T, and B cells and improve the immune function of cyclophosphamide-treated mice.
在这项实验中，从野葛（Willd.）中提取了多糖，并研究了其结构特征和生物活性。结果表明，野葛多糖（PLP）由岩藻糖、阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖以摩尔比 0.09:1.25:2.19:95.74:0.43:0.30 组成，数均分子量（Mn）为 14.463 kDa。此外，傅里叶变换红外光谱（FT-IR）、甲基化和 NMR 分析表明，PLP 主要由主链→4)-α-Glcp（1→和→4,6）-α-Glcp（1→和支链α-Glcp（1→组成。体外实验结果表明，PLP 可刺激 RAW264.7 和（T 和 B）淋巴细胞表面分子的表达和增殖，同时刺激其细胞因子的分泌。体内实验表明，PLP 可改善环磷酰胺诱导免疫抑制小鼠的免疫器官指数、细胞因子含量和 T 淋巴细胞亚群。这些数据证明，PLP 可作为一种有用的免疫调节剂，增强 RAW264.7、T 和 B 细胞的免疫活性，改善环磷酰胺处理小鼠的免疫功能。
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