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环状RNA的RNA测序分析及circPVT1在皮肤鳞状细胞癌中的致癌作用

RNA-Seq Profiling of Circular RNAs and the Oncogenic Role of circPVT1 in Cutaneous Squamous Cell Carcinoma.

作者信息

Chen Shuang, Ding Junli, Wang Yunlin, Lu Tao, Wang Lili, Gao Xinghua, Chen Hongduo, Qu Le, He Chundi

机构信息

Department of Dermatology, No.1 Hospital of China Medical University, Hospital of China Medical University, Key Laboratory of Immunohematology, Shenyang, Liaoning, People's Republic of China.

出版信息

Onco Targets Ther. 2020 Jul 12;13:6777-6788. doi: 10.2147/OTT.S252233. eCollection 2020.

DOI:10.2147/OTT.S252233
PMID:32764965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7367724/
Abstract

BACKGROUND

Cutaneous squamous cell carcinoma (CSCC) is associated with a poor 5-year survival rate. circRNAs have an important role in a number of physiological and pathological processes. However, the relationship between circRNAs and cutaneous squamous cell carcinoma (CSCC) is unclear.

PURPOSE

The aim of the present study was to investigate the expression of circRNAs in cutaneous squamous cell carcinoma (CSCC) and its effect on CSCC proliferation and metastasis.

METHODS

We used high-throughput sequencing (RNA-seq) to identify circRNAs that were differentially in CSCC tissue and their paracarcinoma tissue. Quantitative real-time PCR results confirm deep-sequencing findings in CSCC tissue and cell lines. CCK-8 assay and flow cytometry were used to detect the effect of circPVT1 on the proliferation and migration of CSCC cells.

RESULTS

We identified 449 circRNAs that were differentially expressed between CSCC and normal adjacent tissue samples. circPVT1 (hsa_circ_0001821) was further researched to confirm its oncogene role in CSCC.

CONCLUSION

Differentially expressed circular RNA plays an important role in the development of CSCC, and circPVT1 may be an important target for the treatment of CSCC.

摘要

背景

皮肤鳞状细胞癌（CSCC）的5年生存率较低。环状RNA（circRNAs）在许多生理和病理过程中发挥重要作用。然而，circRNAs与皮肤鳞状细胞癌（CSCC）之间的关系尚不清楚。

目的

本研究旨在探讨circRNAs在皮肤鳞状细胞癌（CSCC）中的表达及其对CSCC增殖和转移的影响。

方法

我们使用高通量测序（RNA-seq）来鉴定CSCC组织及其癌旁组织中差异表达的circRNAs。定量实时PCR结果证实了CSCC组织和细胞系中的深度测序结果。采用CCK-8法和流式细胞术检测circPVT1对CSCC细胞增殖和迁移的影响。

结果

我们鉴定出449个在CSCC与正常相邻组织样本之间差异表达的circRNAs。对circPVT1（hsa_circ_0001821）进行进一步研究，以证实其在CSCC中的癌基因作用。

结论

差异表达的环状RNA在CSCC的发生发展中起重要作用，circPVT1可能是CSCC治疗的重要靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/15e3a8ccd9e2/OTT-13-6777-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/2ac5c5f1e513/OTT-13-6777-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/ca44b22e4e1b/OTT-13-6777-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/1109d087f298/OTT-13-6777-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/52648ff3d24c/OTT-13-6777-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/a9e38d528568/OTT-13-6777-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/15e3a8ccd9e2/OTT-13-6777-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/2ac5c5f1e513/OTT-13-6777-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/ca44b22e4e1b/OTT-13-6777-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/1109d087f298/OTT-13-6777-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/52648ff3d24c/OTT-13-6777-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/a9e38d528568/OTT-13-6777-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/7367724/15e3a8ccd9e2/OTT-13-6777-g0006.jpg

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1
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2
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Nat Commun. 2019 Jul 19;10(1):3200. doi: 10.1038/s41467-019-11162-4.
3
Circbank: a comprehensive database for circRNA with standard nomenclature.Circbank：一个具有标准命名法的 circRNA 综合数据库。
circ_0001821 通过调控 microRNA-148a-3p/EGFR 轴和激活磷脂酰肌醇 3-激酶/ Akt 通路促进皮肤鳞状细胞癌的发展。
Mol Cell Biol. 2022 Mar 17;42(3):e0008921. doi: 10.1128/MCB.00089-21. Epub 2022 Feb 22.
4
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Am J Cancer Res. 2021 Nov 15;11(11):5591-5608. eCollection 2021.
5
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Front Oncol. 2021 Nov 4;11:762960. doi: 10.3389/fonc.2021.762960. eCollection 2021.
6
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Am J Cancer Res. 2021 Oct 15;11(10):4826-4843. eCollection 2021.
7
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5
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6
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7
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