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大肠杆菌K-12 sad基因的克隆与表达

Cloning and expression of the Escherichia coli K-12 sad gene.

作者信息

Marek L E, Henson J M

机构信息

Department of Microbiology, Montana State University, Bozeman 59717.

出版信息

J Bacteriol. 1988 Feb;170(2):991-4. doi: 10.1128/jb.170.2.991-994.1988.

DOI:10.1128/jb.170.2.991-994.1988
PMID:3276667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210754/
Abstract

The Escherichia coli K-12 sad gene, which encodes an NAD-dependent succinic semialdehyde dehydrogenase, was cloned into a high-copy-number vector. Minicells carrying a sad+ plasmid produced a 55,000-dalton peptide, the probable sad gene product.

摘要

编码一种依赖NAD的琥珀酸半醛脱氢酶的大肠杆菌K - 12 sad基因被克隆到一个高拷贝数载体中。携带sad⁺质粒的微小细胞产生了一种55000道尔顿的肽，这可能是sad基因的产物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/210754/2737492c64c3/jbacter00180-0517-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/210754/2737492c64c3/jbacter00180-0517-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a89/210754/2737492c64c3/jbacter00180-0517-a.jpg

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本文引用的文献

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Two succinic semialdehyde dehydrogenases are induced when Escherichia coli K-12 Is grown on gamma-aminobutyrate.当大肠杆菌K-12在γ-氨基丁酸上生长时，会诱导产生两种琥珀酸半醛脱氢酶。
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An Escherichia coli mutant defective in the NAD-dependent succinate semialdehyde dehydrogenase.
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