Wang Weike, Lu Na, Jiang Cheng, Chen Guanping
Hangzhou Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
Cancer Institute of Integrated Traditional Chinese and Western Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China.
PeerJ. 2025 Jan 22;13:e18758. doi: 10.7717/peerj.18758. eCollection 2025.
The objective of the present study is to examine the total phenolic and flavonoid content of an ethanol extract of and to evaluate its phytochemical properties, antioxidant activity, and capacity to protect DNA from damage. This pharmaceutical/food resource mushroom may serve as a novel substitute functional food for health-conscious consumers, given its promising source of phenolics and flavonoids.
ethanol extract (SEE) was evaluated for total phenolic and flavonoid contents, while UPLC-MS analysis was used for terpenoids, phenylpropanoid, flavonoids, steroidal, phenols identification, and function prediction. Antioxidant and anti-DNA damage activities were tested using ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS), and DNA damage protection assay.
Total phenolic content (TPC) in SEE was 385.38 ± 1.36 mg GA/g extract, while total flavonoid content (TFC) was 298.22 ± 2.38 mg QE/g extract. The extracts exhibited high antioxidant and free radical scavenging activities with relatively stronger free radical scavenging activity. A total of 491 metabolites were investigated by Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Most of the top 20 compounds were predicted to have various functions like antioxidant, anti-cancer and anti-inflammatory. This study highlighted was a beneficial source of phenolics and flavonoids. It contains potential natural antioxidant that could be used as a lead contender in the development of antioxidant medicines for the treatment of a wide range of oxidative stress-related illnesses.
本研究的目的是检测[某种物质]乙醇提取物中的总酚和黄酮含量，并评估其植物化学性质、抗氧化活性以及保护DNA免受损伤的能力。鉴于其富含酚类和黄酮类物质，这种药用/食用资源蘑菇可能成为注重健康的消费者的新型替代功能性食品。
对乙醇提取物（SEE）的总酚和黄酮含量进行评估，同时采用超高效液相色谱-质谱联用（UPLC-MS）分析萜类、苯丙素类、黄酮类、甾体类、酚类物质，并进行功能预测。使用铁还原抗氧化能力（FRAP）、1,1-二苯基-2-苦基肼（DPPH）、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）以及DNA损伤保护试验检测抗氧化和抗DNA损伤活性。
SEE中的总酚含量（TPC）为385.38±1.36mg没食子酸/g提取物，总黄酮含量（TFC）为298.22±2.38mg槲皮素当量/g提取物。提取物表现出高抗氧化和自由基清除活性，且自由基清除活性相对较强。通过超高效液相色谱-质谱联用（UPLC-MS）共研究了491种代谢物。预测排名前20的大多数化合物具有多种功能，如抗氧化、抗癌和抗炎。本研究强调[某种物质]是酚类和黄酮类的有益来源。它含有潜在的天然抗氧化剂，可作为开发治疗多种与氧化应激相关疾病的抗氧化药物的有力候选物。
