Zanganeh Elham, Soudi Sara, Zavaran Hosseini Ahmad
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic Address:
Cell J. 2020 Jul;22(Suppl 1):11-18. doi: 10.22074/cellj.2020.6838. Epub 2020 Jul 18.
Leishmaniasis is of public health problems, especially in endemic areas. The activation of macrophages, as the main host of leishmania and promotion of the TH1 immune responses, are the main goal of im-munotherapy methods. Recently, the immunomodulatory role of mesenchymal stem cells (MSCs) in infectious disease has been considered. Different studies demonstrated the immunostimulatory effect of MSCs on macrophages in response to L.major. In this study, the effect of MSCs on cutaneous leishmaniasis in BALB/c mice was assessed.
To do this experimental research, BALB/c mice infected with that was followed by multiple subcutaneous injections of MSCs at infection site at different intervals. Footpad thickness, spleen parasite burden, lymph node, and spleen cytokine production were measured to determine the efficacy of cell therapy.
Significant (P<0.05) reduction in footpad thickness and delayed wound formation was observed in MSCs treated group. The spleen of the MSCs-treated group indicated a two-fold reduction in parasite burden compared with non-treated infected mice. In addition, nitric oxide (NO), interleukin-10 (IL-10), and tumor necrosis factor-alpha (TNF-α) production of lymph node isolated cells and splenocytes changed to the benefit of macrophage activation in response to in MSCs treated group. A two-fold increase in interferon-gamma (IFN-γ) production in the lymph node was determined in the MSCs-treated group.
Although MSCs therapy could not clear the parasite, the results confirm the ability of MSCs to enhance immune responses against leishmania by induction of inflammatory responses and slowing down the spread of parasites. However, further studies needed to improve the efficacy of this method and provide a therapeutic protocol.
利什曼病是一个公共卫生问题，在流行地区尤为如此。激活巨噬细胞（利什曼原虫的主要宿主）并促进TH1免疫反应是免疫治疗方法的主要目标。最近，间充质干细胞（MSCs）在传染病中的免疫调节作用受到了关注。不同研究表明，MSCs对巨噬细胞针对硕大利什曼原虫具有免疫刺激作用。在本研究中，评估了MSCs对BALB/c小鼠皮肤利什曼病的影响。
为进行本实验研究，用[未提及具体感染物]感染BALB/c小鼠，随后在不同时间间隔于感染部位多次皮下注射MSCs。测量足垫厚度、脾脏寄生虫负荷、淋巴结和脾脏细胞因子产生情况以确定细胞治疗的效果。
在MSCs治疗组中观察到足垫厚度显著降低（P<0.05）且伤口形成延迟。与未治疗的感染小鼠相比，MSCs治疗组的脾脏寄生虫负荷降低了两倍。此外，在MSCs治疗组中，分离的淋巴结细胞和脾细胞产生的一氧化氮（NO）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）发生变化，有利于巨噬细胞针对[未提及具体刺激物]的激活。在MSCs治疗组中，淋巴结中干扰素-γ（IFN-γ）的产生增加了两倍。
尽管MSCs治疗不能清除寄生虫，但结果证实了MSCs通过诱导炎症反应和减缓寄生虫传播来增强针对利什曼原虫的免疫反应的能力。然而，需要进一步研究以提高该方法的疗效并提供治疗方案。
