Csángó P A, Sarov B, Schiøtz H, Sarov I
Department of Microbiology, Vest-Agder Central Hospital, Kristiansand, Norway.
J Clin Pathol. 1988 Jan;41(1):89-92. doi: 10.1136/jcp.41.1.89.
The efficiency of an immunoperoxidase serological assay and culture of Chlamydia trachomatis were compared in 127 women seeking first trimester abortion. Serum IgG and IgA antibodies specific for C trachomatis were detected by a single serovar (L2) inclusion immunoperoxidase assay (IPA). Eighty (63%) women were seropositive for chlamydial IgG and 31 (24%) for IgA antibodies. C trachomatis was isolated from 21 of 127 (17%) women. Twenty of the 80 women (25%) seropositive for specific IgG antibodies and one of 47 (2%) patients without these antibodies were culture positive (p less than 0.001). Compared with isolation, chlamydial antibodies at a titre of greater than or equal to 16 showed high sensitivity and negative predictive value (95% and 98%, respectively), but low specificity and efficiency (43% and 52%, respectively). Chlamydial IgA antibodies at a titre of greater than or equal to 8 showed low sensitivity (52%), but a higher specificity, negative predictive value, and efficiency of 81%, 90%, and 76%, respectively. C trachomatis IgG antibodies at a titre of 16 as determined by IPA can be used as an efficient negative exclusion marker for active chlamydial infection in screening women seeking abortion.
对127名寻求孕早期人工流产的女性进行了沙眼衣原体免疫过氧化物酶血清学检测和培养的效率比较。通过单血清型（L2）包涵体免疫过氧化物酶检测法（IPA）检测针对沙眼衣原体的血清IgG和IgA抗体。80名（63%）女性沙眼衣原体IgG血清学阳性，31名（24%）女性IgA抗体血清学阳性。127名女性中有21名（17%）分离出沙眼衣原体。80名特异性IgG抗体血清学阳性的女性中有20名（25%）培养阳性，47名无这些抗体的患者中有1名（2%）培养阳性（p<0.001）。与分离培养相比，滴度大于或等于16的沙眼衣原体抗体显示出高敏感性和阴性预测值（分别为95%和98%），但特异性和效率较低（分别为43%和52%）。滴度大于或等于8的沙眼衣原体IgA抗体敏感性较低（52%），但特异性、阴性预测值和效率较高，分别为81%、90%和76%。通过IPA测定的滴度为16的沙眼衣原体IgG抗体可作为筛查寻求人工流产女性活动性沙眼衣原体感染的有效阴性排除标志物。
