Piura B, Sarov I, Sarov B, Kleinman D, Chaim W, Insler V
Eur J Epidemiol. 1985 Jun;1(2):110-6. doi: 10.1007/BF00141802.
The feasibility of applying elevated Chlamydia trachomatis specific IgG antibody and serum IgA antibodies as a non-invasive screening test for C. trachomatis associated salpingitis was analysed in 54 salpingitis patients and 294 apparently healthy women by the single antigen (L2) immunoperoxidase assay (IPA). The prevalence rate of C. trachomatis IgG antibody (titre greater than or equal to 64) was significantly higher in the salpingitis patients in comparison to control (67% versus 23%). The prevalence rate of elevated C. trachomatis IgG titres (greater than or equal to 128, greater than or equal to 256 and greater than or equal to 512) was significantly higher in the salpingitis patients as compared to the controls. For example, at an IgG titre of greater than or equal to 128 the prevalence rate was 57% in the salpingitis patients and 8% in the healthy controls (p less than 0.0001). The prevalence of C. trachomatis IgA antibodies (titre greater than or equal to 16) was significantly higher in salpingitis patients in comparison to controls (37% versus 4%). The prevalence of elevated IgA titres (greater than or equal to 32 and greater than or equal to 64) was found to be significantly higher in salpingitis patients as compared to controls. All the IgG seropositive salpingitis patients were also found to have C. trachomatis IgG antibodies. It appears that testing for IgG antibodies at a serum dilution of 1:128, and for IgA antibodies at a dilution of 1:16 by the IPA test comprises the best combination for the differentiation between the salpingitis patients and apparently healthy controls, and it is suggested that this be used as a marker of active C. trachomatis infection.
采用单抗原（L2）免疫过氧化物酶试验（IPA），对54例输卵管炎患者和294例表面健康的女性进行分析，以探讨将沙眼衣原体特异性IgG抗体和血清IgA抗体升高作为沙眼衣原体相关性输卵管炎非侵入性筛查试验的可行性。与对照组相比，输卵管炎患者中沙眼衣原体IgG抗体（滴度大于或等于64）的患病率显著更高（67%对23%）。与对照组相比，输卵管炎患者中沙眼衣原体IgG滴度升高（大于或等于128、大于或等于256和大于或等于512）的患病率显著更高。例如，在IgG滴度大于或等于128时，输卵管炎患者的患病率为57%，健康对照组为8%（p小于0.0001）。与对照组相比，输卵管炎患者中沙眼衣原体IgA抗体（滴度大于或等于16）的患病率显著更高（37%对4%）。与对照组相比，发现输卵管炎患者中IgA滴度升高（大于或等于32和大于或等于64）的患病率显著更高。还发现所有IgG血清阳性的输卵管炎患者也都有沙眼衣原体IgG抗体。似乎通过IPA试验在血清稀释度为1:128时检测IgG抗体，在稀释度为1:16时检测IgA抗体，是区分输卵管炎患者和表面健康对照组的最佳组合，建议将其用作活动性沙眼衣原体感染的标志物。
