Sarov I, Lunenfeld E, Sarov B, Hanuka N, Rosenzweig R, Potashnik G, Chaim W, Insler V
Virology Unit, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.
Eur J Epidemiol. 1988 Jun;4(2):216-23. doi: 10.1007/BF00144755.
The prevalence rate of IgG and IgA antibodies to Chlamydia was analyzed in 50 women with laparoscopy-verified tubal infertility and in 50 age-matched control women by single serovar (L2) inclusion immunoperoxidase assay (IPA) and by immunoblotting technique (IB). Women with tubal infertility had significantly (p less than 0.001) elevated IPA Chlamydia IgG antibody titer greater than or equal to 128 and greater than or equal to 256 than controls (64% vs 16%. Odds ratios = 9.3 and 50% vs 10%, Odds ratio = 9 respectively). The prevalence rate of IPA IgA antibody titer (greater than or equal to 16) to Chlamydia was also significantly higher (p less than 0.001) in women with tubal infertility than controls (48% vs 8%, Odds ratio = 10.6). Antibodies to at least 19 chlamydial structural polypeptides ranging in molecular weight from 30 kD to 204 kD, were detected by the IB technique in the IPA seropositive sera. Antibodies to 57-60 kD were detectable in almost all the IPA IgG and IgA seropositive sera. The prevalence rate of IgG antibody to 57 kD-60 kD was significantly higher in women with obstructive infertility than healthy woman (84% vs. 56% p less than 0.01; Odds ratio = 3.8). More significantly, higher differences to 57-60 kD polypeptide were found in the case of IgA between the infertile women and controls (52% vs. 10%, p less than 0.001; Odds ratio = 9.7). The significance of IPA and IB technique for screening of infertile women is discussed.
采用单血清型（L2）包涵体免疫过氧化物酶试验（IPA）和免疫印迹技术（IB），对50例经腹腔镜检查证实为输卵管性不孕的女性及50例年龄匹配的对照女性进行衣原体IgG和IgA抗体患病率分析。输卵管性不孕女性的IPA衣原体IgG抗体滴度显著升高（p<0.001），滴度大于或等于128及大于或等于256的比例高于对照组（分别为64%对16%，优势比=9.3；50%对10%，优势比=9）。输卵管性不孕女性中IPA衣原体IgA抗体滴度（大于或等于16）的患病率也显著高于对照组（p<0.001）（48%对8%，优势比=10.6）。通过IB技术在IPA血清学阳性血清中检测到至少19种衣原体结构多肽的抗体，分子量范围为30kD至204kD。在几乎所有IPA IgG和IgA血清学阳性血清中均可检测到针对57 - 60kD的抗体。梗阻性不孕女性中针对57kD - 60kD的IgG抗体患病率显著高于健康女性（84%对56%，p<0.01；优势比=3.8）。更显著的是，不育女性与对照组之间在针对57 - 60kD多肽的IgA方面差异更大（52%对10%，p<0.001；优势比=9.7）。文中讨论了IPA和IB技术在筛查不孕女性中的意义。
