a Beijing Tsinghua Changgung Hospital, School of Clinical Medicine , Tsinghua University , Beijing , China.
b School of Life Sciences, Tsinghua-Peking Center for Life Sciences , Tsinghua University , Beijing , China.
Artif Cells Nanomed Biotechnol. 2018;46(sup3):S460-S470. doi: 10.1080/21691401.2018.1499662. Epub 2018 Sep 27.
To resolve the problems of bacterial infections and the low efficiency of osteogenesis of implanted titanium alloys in clinical dental and bone therapy, we developed a bifunctional titanium alloy (Ti) with a nano-hydroxyapatite (HA) coating (HBD + BMP/HA-Ti), which enables the sustained release of the natural antimicrobial peptide human β-defensin 3 (HBD-3) and bone morphogenetic protein-2 (BMP-2). Due to the poriferous nano-sized structure of the HA coating with a 20-30 μm thickness, the HBD + BMP/HA-Ti material had a high encapsulation efficiency (>74%) and exhibited synchronized slow release of HBD-3 and BMP-2. In an antibacterial test, HBD + BMP/HA-Ti prevented the growth of bacteria in an inoculated medium, and its surface remained free from viable bacteria after a continuous incubation with Gram-negative and Gram-positive strains for 7 days. Furthermore, good adhesion, proliferation and osteogenic differentiation of hBMSCs in contact with HBD + BMP/HA-Ti were achieved in 7 days. Therefore, the bifunctional titanium alloy HBD + BMP/HA-Ti has a great potential for eventual applications in the protection of implants against bacteria in the orthopaedic and dental clinic.
为了解决临床牙科和骨治疗中植入钛合金的细菌感染和成骨效率低的问题，我们开发了一种具有纳米羟基磷灰石（HA）涂层的双功能钛合金（HBD+BMP/HA-Ti），能够持续释放天然抗菌肽人β防御素 3（HBD-3）和骨形态发生蛋白 2（BMP-2）。由于 HA 涂层具有 20-30μm 厚度的多孔纳米结构，HBD+BMP/HA-Ti 材料具有高包封效率（>74%），并表现出 HBD-3 和 BMP-2 的同步缓慢释放。在抗菌试验中，HBD+BMP/HA-Ti 阻止了接种培养基中细菌的生长，并且在与革兰氏阴性和革兰氏阳性菌连续孵育 7 天后，其表面仍然没有存活的细菌。此外，在 7 天内与 HBD+BMP/HA-Ti 接触的 hBMSCs 实现了良好的黏附、增殖和成骨分化。因此，双功能钛合金 HBD+BMP/HA-Ti 在保护植入物免受骨科和牙科诊所中细菌感染方面具有很大的应用潜力。
