Protective Role of Astrocyte-Derived Exosomal in Cerebral Ischemic-Reperfusion Injury by Regulating the Signaling Pathway and Targeting .

BACKGROUND

Evidence has shown that microRNAs (miRNAs) are implicated in ischemic diseases. Therefore, the aim of the present study was to identify the functions of astrocyte (ATC)-derived exosomal on cerebral ischemic-reperfusion (I/R) injury.

METHODS

A rat model of cerebral I/R injury was initially established, followed by injection of ATC-derived exosomes. Next, the protective function of ATC-derived exosomes in rats with cerebral I/R injury was evaluated, and then the effect of on rats with cerebral I/R injury was evaluated by changing expression in exosomes. PC12 cells that underwent oxygen-glucose deprivation/reoxygenation were used to simulate I/R in vitro. The effect of ATC-derived exosomal on the viability and apoptosis of OGD/R-treated PC12 cells was also assessed. The bioinformatic analysis predicted the targeted gene of .

RESULTS

It was found that I/R was damaging to the brain nerves of rats, while ATC-derived exosomal relieved nerve damage caused by I/R. Furthermore, the in vitro experiments demonstrated that ATC-derived exosomal increased OGD/R-inhibited PC12 cell activity and suppressed cell apoptosis. Bioinformatics predicted that targeted cathepsin B (). upregulation blocked the protective roles of . In addition, was found to downregulate the signaling pathway by targeting .

CONCLUSION

The present study demonstrated that ATC-derived exosomal alleviates nerve damage in rats with cerebral I/R injury by targeting and downregulating the pathway. This may offer novel insights into treatment for I/R injury.