Ederveen Thomas H A, Smits Jos P H, Boekhorst Jos, Schalkwijk Joost, van den Bogaard Ellen H, Zeeuwen Patrick L J M
Center for Molecular and Biomolecular Informatics (CMBI), Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Medical Center (Radboudumc), Nijmegen, The Netherlands.
Department of Dermatology, RIMLS, Radboudumc, Nijmegen, The Netherlands.
J Dermatol. 2020 Oct;47(10):1110-1118. doi: 10.1111/1346-8138.15536. Epub 2020 Aug 17.
Microbiota live in a closely regulated interaction with their environment, and vice versa. The presence and absence of microbial entities is greatly influenced by features of the niche in which they thrive. Characteristic of this phenomenon is that different human skin sites harbor niche-specific communities of microbes. Microbial diversity is considerable, and the current challenge lies in determining which microbes and (corresponding) functionality are of importance to a given ecological niche. Furthermore, as there is increasing evidence of microbial involvement in health and disease, the need arises to fundamentally understand microbiome processes for application in health care, nutrition and personal care products (e.g. diet, cosmetics, probiotics). This review provides a current overview of state-of-the-art sequencing-based techniques and corresponding data analysis methodology for profiling of complex microbial communities. Furthermore, we also summarize the existing knowledge regarding cutaneous microbiota and their human host for a wide range of skin diseases.
微生物群与其生存环境处于密切调控的相互作用之中，反之亦然。微生物实体的存在与否受到它们所生存的生态位特征的极大影响。这一现象的特点是，人类不同的皮肤部位存在着特定生态位的微生物群落。微生物多样性十分可观，当前的挑战在于确定哪些微生物及其（相应的）功能对于特定的生态位至关重要。此外，鉴于越来越多的证据表明微生物与健康和疾病有关，因此有必要从根本上了解微生物组过程，以便应用于医疗保健、营养和个人护理产品（如饮食、化妆品、益生菌）。本综述提供了基于测序的最新技术以及用于分析复杂微生物群落的相应数据分析方法的当前概述。此外，我们还总结了有关多种皮肤疾病的皮肤微生物群及其人类宿主的现有知识。
