环状 RNA hsa_circ_01844 的上调诱导脑胶质瘤细胞凋亡，并抑制其增殖和迁移。
Up-regulation of circular RNA hsa_circ_01844 induces apoptosis and suppresses proliferation and migration of glioblastoma cells.
机构信息
Department of Neurosurgery, The 904th Hospital of Joint Logistic Support Force PLA, Wuxi, Jiangsu 214000, China.
Wuxi Clinical College of Anhui Medical University, Wuxi, Jiangsu 214000, China.
出版信息
Chin Med J (Engl). 2020 Aug 13;134(1):81-87. doi: 10.1097/CM9.0000000000000979.
BACKGROUND
Previous studies have demonstrated that various circular RNAs are involved in the malignant proliferation of cancers, such as liver cancer, lung cancer, breast cancer, and others. The potential role of circular RNAs in glioblastoma, however, is still uncertain. In this study, we aimed to study the potential role of hsa_circ_01844 in glioblastoma.
METHODS
Using reverse transcription-polymerase chain reaction (RT-PCR) method, hsa_circ_01844 expression was measured in five glioblastoma samples and five normal brain samples. To evaluate the potential function of hsa_circ_01844 in glioblastoma, hsa_circ_01844 was overexpressed in glioblastoma cell lines (U251 and U87 cells). Using these two cell lines, in vitro experiments including the flow cytometry assay, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, Transwell assay, and cell apoptosis assay were performed to investigate the role of hsa_circ_01844 in glioblastoma. Student t test and one-way analysis of variance were used for statistical analysis.
RESULTS
The expression of circular RNA hsa_circ_01844 was lower in glioblastoma tissues when compared with the normal brain tissues by RT-PCR method (0.034 ± 0.036 vs. 1.630 ± 0.891, P < 0.001). Using two glioblastoma cell lines, we found that overexpression of hsa_circ_01844 in glioblastoma cells suppressed their proliferation, colony formation, migration, and increased the apoptotic rate compared with empty vector group and blank control group (all P < 0.05).
CONCLUSION
Hsa_circ_01844 shows decreased expression in glioblastoma and its overexpression induces apoptosis and inhibits proliferation, migration, and invasion of glioblastoma cells.
背景
先前的研究表明，多种环状 RNA 参与了肝癌、肺癌、乳腺癌等癌症的恶性增殖。然而，环状 RNA 在胶质母细胞瘤中的潜在作用尚不确定。在本研究中，我们旨在研究 hsa_circ_01844 在胶质母细胞瘤中的潜在作用。
方法
采用逆转录-聚合酶链反应（RT-PCR）方法，检测 5 例胶质母细胞瘤样本和 5 例正常脑组织样本中 hsa_circ_01844 的表达。为了评估 hsa_circ_01844 在胶质母细胞瘤中的潜在功能，在胶质母细胞瘤细胞系（U251 和 U87 细胞）中转染 hsa_circ_01844。利用这两种细胞系，通过流式细胞术、3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）比色法、Transwell 实验和细胞凋亡实验进行体外实验，以研究 hsa_circ_01844 在胶质母细胞瘤中的作用。采用 Student t 检验和单因素方差分析进行统计学分析。
结果
RT-PCR 方法显示，与正常脑组织相比，胶质母细胞瘤组织中环状 RNA hsa_circ_01844 的表达较低（0.034±0.036 比 1.630±0.891，P<0.001）。使用两种胶质母细胞瘤细胞系，我们发现与空载体组和空白对照组相比，过表达 hsa_circ_01844 可抑制胶质母细胞瘤细胞的增殖、集落形成、迁移，并增加细胞凋亡率（均 P<0.05）。
结论
hsa_circ_01844 在胶质母细胞瘤中表达下调，过表达 hsa_circ_01844 可诱导胶质母细胞瘤细胞凋亡，并抑制其增殖、迁移和侵袭。
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