三种双歧杆菌共生关系可增加人肠道微生物群对唾液酸化免疫球蛋白 G 的发酵。

Commensal Relationship of Three Bifidobacterial Species Leads to Increase of Fermentation of Sialylated Immunoglobulin G by Human Gut Microbiota.

机构信息

College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.

College of Food Engineering, Anhui Science and Technology University, Fengyang 233100, Anhui, China.

出版信息

J Agric Food Chem. 2020 Aug 26;68(34):9110-9119. doi: 10.1021/acs.jafc.0c03628. Epub 2020 Aug 13.

DOI:10.1021/acs.jafc.0c03628

PMID:32806107

Abstract

Sialylated immunoglobulin G (IgG) is an important immunoglobulin in breast milk, but its effect on adult gut microbiota is not yet clear due to digestion by pepsin. Based on our previous IgG protecting study, effects of sialylated IgG on gut microbiota were investigated by anaerobic fermentation in the present study. It was found that the addition of sialylated IgG could significantly promote the growth of . Meanwhile, three bifidobacterial species CCX 19061, CCX 19041, and subsp. CCX 19042 were isolated. Furthermore, CCX 19041 and subsp. CCX 19042 showed co-culture growth property with CCX 19061 in a sialylated IgG-based medium, which was also supported by changes of free monosaccharides and -glycan structure. These findings suggest that the increase of fermentation is attributed to the commensal relationship of the three bifidobacterial species by utilizing sugars released from sialylated IgG.

摘要

唾液酸化免疫球蛋白 G（IgG）是母乳中的一种重要免疫球蛋白，但由于胃蛋白酶的消化，其对成人肠道微生物群的影响尚不清楚。基于我们之前的 IgG 保护研究，本研究通过厌氧发酵研究了唾液酸化 IgG 对肠道微生物群的影响。结果发现，添加唾液酸化 IgG 可显著促进的生长。同时，分离到三种双歧杆菌 CCX 19061、CCX 19041 和 subsp. CCX 19042。此外，CCX 19041 和 subsp. CCX 19042 与 CCX 19061 在基于唾液酸化 IgG 的培养基中表现出共培养生长特性，这也得到游离单糖和 -聚糖结构变化的支持。这些发现表明，的发酵增加归因于三种双歧杆菌通过利用从唾液酸化 IgG 释放的糖而产生的共生关系。