Lee Myung-Jin, Kim Ji-Yeong, Seo Ji-Young, Mangal Utkarsh, Cha Jung-Yul, Kwon Jae-Sung, Choi Sung-Hwan
Division of Health Science, Department of Dental Hygiene, Baekseok University, Cheonan 31065, Korea.
Department of Orthodontics, Institute of Craniofacial Deformity, Yonsei University College of Dentistry, Seoul 03722, Korea.
Nanomaterials (Basel). 2020 Aug 12;10(8):1581. doi: 10.3390/nano10081581.
Since pits and fissures are the areas most commonly affected by caries due to their structural irregularity, bioactive resin-based sealant (RBS) may contribute to the prevention of secondary caries. This study aims to investigate the mechanical, physical, ion-release, enamel remineralisation, and antibacterial capabilities of the novel RBS with bioactive glass (BAG) and 2-methacryloyloxyethyl phosphorylcholine (MPC). For the synthesis, 12.5 wt% BAG and 3 wt% MPC were incorporated into RBS. The contact angle, flexural strength, water sorption, solubility, and viscosity were investigated. The release of multiple ions relating to enamel remineralisation was investigated. Further, the attachments of bovine serum albumin, brain heart infusion broth, and on RBS were studied. Finally, the thickness and biomass of a human saliva-derived microsm biofilm model were analysed before aging, with static immersion aging and with thermocycling aging. In comparison to commercial RBS, BAG+MPC increased the wettability, water sorption, solubility, viscosity, and release of multiple ions, while the flexural strength did not significantly differ. Furthermore, RBS with MPC and BAG+MPC significantly reduced protein and bacteria adhesion and suppressed multi-species biofilm attachment regardless of the existence of aging and its type. The novel RBS has great potential to facilitate enamel remineralisation and suppress biofilm adhesion, which could prevent secondary dental caries.
由于窝沟因其结构不规则而成为龋齿最常累及的区域，基于生物活性树脂的封闭剂（RBS）可能有助于预防继发龋。本研究旨在探究含有生物活性玻璃（BAG）和2-甲基丙烯酰氧乙基磷酰胆碱（MPC）的新型RBS的机械性能、物理性能、离子释放性能、牙釉质再矿化能力及抗菌能力。合成时，将12.5 wt%的BAG和3 wt%的MPC加入到RBS中。研究了接触角、弯曲强度、吸水性、溶解性和粘度。研究了与牙釉质再矿化相关的多种离子的释放情况。此外，还研究了牛血清白蛋白、脑心浸液肉汤在RBS上的附着情况。最后，分析了人唾液来源的微生态生物膜模型在老化前、静态浸泡老化和热循环老化后的厚度和生物量。与市售RBS相比，BAG+MPC提高了润湿性、吸水性、溶解性、粘度和多种离子的释放量，而弯曲强度无显著差异。此外，含有MPC和BAG+MPC的RBS显著降低了蛋白质和细菌的粘附，并抑制了多种生物膜的附着，无论老化与否及其类型如何。这种新型RBS在促进牙釉质再矿化和抑制生物膜粘附方面具有巨大潜力，这可能预防继发龋齿。
