Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul, Republic of Korea; Department of Orthodontics, Institute of Craniofacial Deformity, Yonsei University College of Dentistry, Seoul, Republic of Korea.
Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul, Republic of Korea; BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
Dent Mater. 2019 Sep;35(9):1331-1341. doi: 10.1016/j.dental.2019.06.004. Epub 2019 Jul 16.
This study evaluated the synergetic effect between surface pre-reacted glass-ionomer (SPRG) filler and 2-methacryloyloxyethyl phosphorylcholine (MPC), for inhibiting multi-species biofilm formation, while maintaining or even improving the original beneficial features of SPRG-filled resin-based composite (RBC).
MPC (1.5-10wt%) was incorporated into commercial SPRG-filled RBC. Then, the inherent properties of RBC, and ion release and acid-neutralising properties associated with SPRG were investigated. Further, protein adsorptions and bacterial adhesion and viability on the SPRG-filled RBC surfaces were studied using four kinds of oral bacteria; Streptococcus mutans, Actinomyces naeslundii, Veillonella parvula, and Porphyromonas gingivalis. Finally, the thickness and biomass of the human saliva-derived biofilm model cultured on test and control samples were analysed.
Addition of MPC content resulted in decreased flexural strength and wettability of SPRG-filled RBC. SPRG-filled RBC released significantly higher amounts of multiple ions as contents of MPC increased. Meanwhile, SPRG-filled RBC with 5-wt% MPC significantly improved acid-neutralising properties than those of other test and control samples (P<0.001). SPRG-filled RBC with 3wt% MPC significantly reduced the amount of adsorbed bovine serum albumin and proteins from the brain heart infusion medium as compared to the control (P<0.01). A similar trend was observed in the attachment of four types of bacteria and multi-species biofilm (P<0.01).
Despite limitation in terms of deteriorations of some physical properties, addition of 3% MPC to SPRG-filled RBC leads to inhibition of the attachment of multi-species bacteria on its surface, as well as inhibition of biofilm growth. Moreover, the original important bioactive features of SPRG-filled RBC such as ion release and acid neutralisations are either maintained or improved upon adding MPC.
本研究评估了表面预反应玻璃离子（SPRG）填料与 2-甲基丙烯酰氧乙基磷酸胆碱（MPC）之间的协同作用，以抑制多种物种生物膜的形成，同时保持甚至改善含 SPRG 的树脂基复合材料（RBC）的原始有益特性。
将 MPC（1.5-10wt%）掺入商业 SPRG 填充 RBC。然后，研究了 RBC 的固有特性以及与 SPRG 相关的离子释放和酸中和特性。进一步研究了四种口腔细菌（变形链球菌、内氏放线菌、小韦荣球菌和牙龈卟啉单胞菌）在 SPRG 填充 RBC 表面的蛋白质吸附和细菌粘附和活力。最后，分析了在测试和对照样品上培养的人唾液衍生生物膜模型的厚度和生物量。
添加 MPC 含量会降低 SPRG 填充 RBC 的弯曲强度和润湿性。随着 MPC 含量的增加，SPRG 填充 RBC 释放出的多种离子的量显著增加。同时，与其他测试和对照样品相比，含 5wt%MPC 的 SPRG 填充 RBC 的酸中和性能显著提高（P<0.001）。与对照相比，含 3wt%MPC 的 SPRG 填充 RBC 显著减少了牛血清白蛋白和脑心浸液培养基中蛋白质的吸附量（P<0.01）。四种细菌和多物种生物膜的附着也观察到类似的趋势（P<0.01）。
尽管某些物理性质恶化受到限制，但向 SPRG 填充 RBC 中添加 3%的 MPC 可抑制多种细菌在其表面的附着，并抑制生物膜的生长。此外，在添加 MPC 后，SPRG 填充 RBC 的原始重要生物活性特性，如离子释放和酸中和，得以维持或改善。
