Department of Microbiology, Foch Hospital, 92150, Suresnes, France.
Department of Pneumonology, Foch Hospital, 92150, Suresnes, France.
Eur J Clin Microbiol Infect Dis. 2021 Feb;40(2):391-395. doi: 10.1007/s10096-020-04007-2. Epub 2020 Aug 17.
Identification of moulds is crucial for the clinical management of patients. The goal of this study was to evaluate the new ID-FUNGI plate (IDFP) for the identification of moulds by MALDI Biotyper. IDFP was compared with Sabouraud with gentamicin and chloramphenicol plate (SAB) for the identification of 80 moulds from respiratory samples and eight reference strains. With the direct transfer method, species identification rose from 6% with SAB to 68% with IDFP using score cut-off 2 and from 20 to 75% using cut-off 1.7 (p < 0.001). Our study highlights that the new IDFP improves mycological diagnostic and workflow in laboratories.
鉴定霉菌对于患者的临床治疗至关重要。本研究旨在通过 MALDI Biotyper 评估新型 ID-FUNGI 板（IDFP）对霉菌的鉴定能力。将 IDFP 与含庆大霉素和氯霉素的沙氏葡萄糖琼脂（SAB）平板比较，用于鉴定 80 株呼吸道样本霉菌和 8 株参考菌株。采用直接转移法，使用 2 分的分界值，IDFP 比 SAB 的菌种鉴定率从 6%上升至 68%；使用 1.7 分的分界值，菌种鉴定率从 20%上升至 75%（p < 0.001）。本研究表明新型 IDFP 可提高实验室的微生物诊断和工作流程效率。
