Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, 130118, Jilin, China.
College of Plant Protection, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Arch Virol. 2020 Nov;165(11):2689-2693. doi: 10.1007/s00705-020-04781-6. Epub 2020 Aug 18.
Here, we describe a novel mycovirus, Auricularia heimuer fusarivirus 1 (AhFV1), isolated from the edible fungus Auricularia heimuer strain CCMJ1296. The virus has a single-stranded positive-sense [+ssRNA] genome of 7,127 nucleotides containing two overlapping open reading frames (ORFs) and a poly(A) tail. The large ORF1 encodes a polyprotein of 1,637 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and DEAD-like helicase superfamily (DEXDc) domains. ORF2 encodes a putative 633-aa protein with unknown function. A BLAST search showed that the nucleotide sequence of the AhFV1 genome is 41.28% identical to that of Sclerotium rolfsii fusarivirus 2 and 40.49% identical to that of Sclerotium rolfsii fusarivirus 1. Phylogenetic analysis based on RdRp and helicase (Hel) sequences indicated that AhFV1 is related to unclassified mycoviruses and other fusariviruses. Our data suggest that AhFV1 should be classified as a member of the newly proposed family "Fusariviridae". This is the second virus and the first full genome sequence of a fusarivirus from A. heimuer.
在这里，我们描述了一种从食用真菌银耳菌株 CCMJ1296 中分离得到的新型真菌病毒，银耳 Heimuer 病毒 1（AhFV1）。该病毒具有 7127 个核苷酸的单链正链 [+ssRNA] 基因组，包含两个重叠的开放阅读框（ORF）和一个 poly(A) 尾巴。大 ORF1 编码一个由 1637 个氨基酸（aa）组成的多蛋白，具有保守的 RNA 依赖性 RNA 聚合酶（RdRp）和 DEAD 样解旋酶超家族（DEXDc）结构域。ORF2 编码一个具有未知功能的推定 633-aa 蛋白。BLAST 搜索显示，AhFV1 基因组的核苷酸序列与 Sclerotium rolfsii fusarivirus 2 的同源性为 41.28%，与 Sclerotium rolfsii fusarivirus 1 的同源性为 40.49%。基于 RdRp 和解旋酶（Hel）序列的系统发育分析表明，AhFV1 与未分类的真菌病毒和其他 fusariviruses 有关。我们的数据表明，AhFV1 应被归类为新提出的家族“Fusariviridae”的成员。这是从银耳中分离得到的第二种病毒和第一种 fusarivirus 的全基因组序列。
