Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Guizhou Key Laboratory of Edible fungi breeding, Guizhou Academy of Agricultural Sciences, Guiyang, 550006, Guizhou, China.
Arch Virol. 2022 Nov;167(11):2223-2227. doi: 10.1007/s00705-022-05540-5. Epub 2022 Aug 13.
Here, we report the characterization of a novel (-)ssRNA mycovirus isolated from Auricularia heimuer CCMJ1222, using a combination of RNA-seq, reverse transcription polymerase chain reaction, 5' and 3' rapid amplification of cDNA ends, and Sanger sequencing. Based on database searches, sequence alignment, and phylogenetic analysis, we designated the virus as "Auricularia heimuer negative-stranded RNA virus 1" (AhNsRV1). This virus has a monopartite RNA genome related to mymonaviruses (order Mononegavirales). The AhNsRV1 genome consists of 11,441 nucleotides and contains six open reading frames (ORFs). The largest ORF encodes a putative RNA-dependent RNA polymerase; the other ORFs encode hypothetical proteins with no conserved domains or known function. AhNsRV1 is the first (-)ssRNA virus and the third virus known to infect A. heimuer.
在这里，我们使用 RNA-seq、逆转录聚合酶链反应、5' 和 3' 快速扩增 cDNA 末端和 Sanger 测序的组合，报告了一种从 Auricularia heimuer CCMJ1222 中分离出的新型（-）ssRNA 真菌病毒的特征。基于数据库搜索、序列比对和系统发育分析，我们将该病毒命名为“银耳负链 RNA 病毒 1”（AhNsRV1）。该病毒具有与单负股病毒（单股负股病毒目）相关的单份 RNA 基因组。AhNsRV1 基因组由 11441 个核苷酸组成，包含六个开放阅读框（ORFs）。最大的 ORF 编码一个假定的 RNA 依赖性 RNA 聚合酶；其他 ORFs 编码没有保守结构域或已知功能的假设蛋白。AhNsRV1 是第一个（-）ssRNA 病毒，也是已知感染银耳的第三种病毒。
