Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha, 410128, Hunan, People's Republic of China.
Arch Virol. 2021 Jul;166(7):2063-2067. doi: 10.1007/s00705-021-05105-y. Epub 2021 May 13.
A novel mycovirus belonging to the proposed family "Fusariviridae" was discovered in Alternaria solani by sequencing a cDNA corresponding to double-stranded RNA extracted from this phytopathogenic fungus. The virus was tentatively named "Alternaria solani fusarivirus 1" (AsFV1). AsFV1 has a single-stranded positive-sense (+ssRNA) genome of 6845 nucleotides containing three open reading frames (ORFs) and a poly(A) tail. The largest ORF, ORF1, encodes a large polypeptide of 1,556 amino acids (aa) with conserved RNA-dependent RNA polymerase and helicase domains. The ORF2 and ORF3 have overlapping regions, encoding a putative protein of 522 amino acids (aa) and a putative protein of 105 amino acids (aa), respectively, both of unknown function. A multiple sequence alignment and phylogenetic analysis revealed that AsFV1 could be a new member of the "Fusariviridae". This is the first report of the full-length nucleotide sequence of a fusarivirus that infects Alternaria solani.
一种新型的真菌病毒属于拟议的“Fusariviridae”家族，通过对从这种植物病原真菌中提取的双链 RNA 进行 cDNA 测序，在链格孢菌中发现了该病毒。该病毒被暂时命名为“Alternaria solani fusarivirus 1”（AsFV1）。AsFV1 具有单链正链（+ssRNA）基因组，长 6845 个核苷酸，包含三个开放阅读框（ORF）和一个 poly(A)尾巴。最大的 ORF1 编码一个由 1556 个氨基酸组成的大多肽，具有保守的 RNA 依赖的 RNA 聚合酶和解旋酶结构域。ORF2 和 ORF3 有重叠区域，分别编码一个推定的 522 个氨基酸（aa）和一个推定的 105 个氨基酸（aa）的蛋白，其功能未知。多重序列比对和系统发育分析表明，AsFV1 可能是“Fusariviridae”的一个新成员。这是首例报道感染链格孢菌的 fusarivirus 的全长核苷酸序列。
