Rutter D A, Ashworth L A, Day A, Funnell S, Lovell F, Robinson A
PHLS Centre for Applied Microbiology and Research, Salisbury, Wilts, UK.
Vaccine. 1988 Feb;6(1):29-32. doi: 10.1016/0264-410x(88)90010-2.
Immunogenicity and reactogenicity of a new acellular pertussis vaccine were tested in healthy adults. The vaccine contained three constituents of Bordetella pertussis; filamentous haemagglutinin, pertussis toxin (PT) and fimbriae bearing agglutinogens 2 and 3. The constituents were separately purified, treated with formaldehyde and combined with one of two aluminium adjuvants. Subjects received one dose of vaccine or an appropriate adjuvant-only preparation and were monitored for clinical responses for 7 days. Results with the two forms of vaccine were similar. Of 35 vaccinees, none had a temperature higher than 37 degrees C or a severe reaction, one had a moderate reaction (possibly due in part to intercurrent infection) and nine had mild reactions confined to localized discomfort and/or erythema or induration at the injection site. All vaccinees had good serum antibody responses to vaccine antigens measured by ELISA and for PT, by neutralization of its effects on Chinese hamster ovary cells.
在健康成年人中测试了一种新型无细胞百日咳疫苗的免疫原性和反应原性。该疫苗包含百日咳博德特氏菌的三种成分；丝状血凝素、百日咳毒素（PT）以及携带凝集原2和3的菌毛。这些成分分别进行纯化，用甲醛处理，然后与两种铝佐剂之一混合。受试者接种一剂疫苗或仅接种适当的佐剂制剂，并监测7天的临床反应。两种形式疫苗的结果相似。在35名接种疫苗者中，无人体温高于37摄氏度或出现严重反应，1人出现中度反应（可能部分归因于并发感染），9人出现轻度反应，仅限于注射部位的局部不适和/或红斑或硬结。通过ELISA法检测，所有接种疫苗者对疫苗抗原均有良好的血清抗体反应，对于PT，通过中和其对中国仓鼠卵巢细胞的作用来检测。
