Ide-Pérez Martín R, Fernández-López Maikel Gilberto, Sánchez-Reyes Ayixon, Leija Alfonso, Batista-García Ramón Alberto, Folch-Mallol Jorge Luis, Sánchez-Carbente María Del Rayo
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mexico.
Centro de Investigación en Dinámica Celular-Instituto de Investigaciones Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mexico.
J Fungi (Basel). 2020 Aug 14;6(3):135. doi: 10.3390/jof6030135.
Since Aromatic hydrocarbons are recalcitrant and toxic, strategies to remove them are needed. The aim of this work was to isolate fungi capable of using aromatic hydrocarbons as carbon sources. Two isolates from an oil polluted site in Mexico were identified through morphological and molecular markers as a novel sp. and an sp. Both strains were able to grow in a wide range of pH media, from 4 to 12, showing their optimal growth at alkaline pH's and are both halotolerant. The strain switched from hyphae to yeast morphotype in high salinity conditions. To the best of our knowledge, this is the first report of salt triggering dimorphism. The strain, which is likely a new undescribed species, was capable of removing singled ringed aromatic compounds such as benzene, xylene, and toluene, but could not remove benzo[] pyrene nor phenanthrene. Nevertheless, these hydrocarbons did not impair its growth. The strain showed a different removal capacity. It could remove the polyaromatic hydrocarbons but performed poorly at removing toluene and xylene. Nevertheless, it still could grow well in the presence of the aromatic compounds. These strains could have a potential for aromatic compounds removal.
由于芳香烃具有顽固性和毒性，因此需要采取去除它们的策略。这项工作的目的是分离能够利用芳香烃作为碳源的真菌。通过形态学和分子标记，从墨西哥一个受石油污染的地点鉴定出两株分离菌，分别为一个新的种和一个种。两株菌株都能够在pH值范围为4至12的多种培养基中生长，在碱性pH值下显示出最佳生长状态，并且都具有耐盐性。菌株在高盐条件下从菌丝形态转变为酵母形态。据我们所知，这是盐引发二态性的首次报道。菌株可能是一个未描述的新物种，能够去除单环芳香化合物，如苯、二甲苯和甲苯，但不能去除苯并[]芘和菲。然而，这些碳氢化合物并未损害其生长。菌株表现出不同的去除能力。它可以去除多环芳烃，但在去除甲苯和二甲苯方面表现不佳。然而，在芳香化合物存在的情况下，它仍然可以良好生长。这些菌株在去除芳香化合物方面具有潜力。
